Kathleen Pratt

Professor and Vice Chair for Research, Department of Medicine

Accepting PhD Students

PhD projects

blood coagulation, anti-drug antibodies, immunothrombosis in COVID-19

Calculated based on the number of publications stored in Pure and citations from PlumX
1986 …2024

Research activity per year

Personal profile

Research interests

Dr. Pratt’s laboratory employs a multidisciplinary approach to studies at the interface between blood coagulation, inflammation and immunology. Early work consisted of molecular modeling and x-ray crystallographic studies. These included determination of high-resolution crystal structures that elucidated the mechanism of fibrin polymerization and the orientation of coagulation factor VIII (FVIII) on activated platelet surfaces. She has studied the structure, function and immunogenicity of blood FVIII for over 20 years. Her studies of alloimmune responses to FVIII encompass structure/function studies, investigations of cellular immune responses to FVIII, mapping of T-cell and B-cell epitopes using multiple techniques, RNASeq/transcriptomics and TCR repertoire analysis of PBMCs and specific lymphocyte populations, and characterization of T-cell clones and polyclonal lines from patients as well as hemophilic mice.

Recent work has included analysis of the national “My Life Our Future” database for hemophilia A and a natural history study analyzing antibody responses in ~400 hemophilia A patients. Results revealed that African American and Hispanic patients have a higher risk of developing neutralizing anti-FVIII antibodies, and ongoing wet-lab studies are revealing mechanisms contributing to this risk. Among these, we recently characterized CD4 T-cell responses to FVIII epitopes in subjects with severe hemophilia A due to an intron-22 inversion mutation (Gunasekera et al., Frontiers in Immunology, in press). We are collaborating with Dr. Margaret Ragni (U. of Pittsburgh) on a natural history study of neutralizing anti-FVIII antibodies in hemophilia A that will include analysis of samples collected during the initial development of these deleterious anti-drug antibody responses. Results will also be relevant to studies of other unwanted anti-drug antibody responses.

In collaboration with Dr. David Scott (USU), we are developing a hemophilia A mouse model transgenic for a FVIII-specific B-cell receptor, which will be used as an in vivo model to study FVIII-specific immune responses and various tolerogenic strategies.

In 2020, our laboratory pivoted in response to the COVID-19 pandemic. Current studies include profiling of plasma, serum and cellular fractions from hospitalized COVID-19 patients, focusing particularly on biomarkers and mechanisms of immunothrombosis. Additional collaborative studies of “long-covid” patient samples will launch in 2023, in collaboration with USU investigators Dr. Mark Haigney, COL David Saunders and their groups.

Another recently funded project addresses a fundamental need in trauma resuscitation: patients who experience substantial blood loss require transfusions with blood from appropriately matched donors to avoid transfusion reactions (e.g. to ABO and Rh antigens on red blood cells and other blood components). Plasma, platelets and/or packed red blood cells are also needed. In collaboration wth Dr. Mark Osborn (U of Minnesota) we will develop "off-the-shelf" resuscitation fluids as potential alternatives to blood from live donors. Techniques will include engineering of red blood cells and platelets grown in cell culture and testing of alternatives to plasma as a carrier solution. These alternative components and solutions will be compared to whole blood, plasma, etc. using functional assays. Dr. Steve Dumler's group (USU) are local collaborators on this interesting project.   


Education/Academic qualification

Biophysical Chemistry, PhD, Cornell University


Award Date: 1 Jan 1992

Mathematical Sciences, Bachelor, University of Washington


Award Date: 1 Jun 1984

Liberal Arts, Bachelor, The Evergreen State College, Olympia, WA


Award Date: 1 Jan 1976

External positions

Interim Vice Chair for Research, Department of Medicine

Jan 2020Jul 2020

Associate Professor, Department of Medicine, tenured


Associate Professor, Department of Medicine, Uniformed Services University of the Health Sciences


Associate Professor, Molecular and Cellular Biology (Secondary)


Affiliate Investigator, Benaroya Research Institute at Virginia Mason


Assistant Professor


Assistant Research Professor, Division of Hematology, University of Washington School of Medicine


Assistant Member, Puget Sound Blood Center


Research Scientist, Puget Sound Blood Center


Staff Scientist, Fred Hutchinson Cancer Research Center


Postdoctoral fellow, University of Washington


Postdoctoral fellow, University of Colorado Boulder


Research Technician, University of Washington School of Medicine



Dive into the research topics where Kathleen Pratt is active. These topic labels come from the works of this person. Together they form a unique fingerprint.
  • 1 Similar Profiles

Collaborations and top research areas from the last five years

Recent external collaboration on country/territory level. Dive into details by clicking on the dots or