A chemically defined liquid medium that supports primary isolation of Rochalimaea (Bartonella) henselae from blood and tissue specimens

M. T. Wong, D. C. Thornton, R. C. Kennedy, M. J. Dolan*

*Corresponding author for this work

Research output: Contribution to journalComment/debate

37 Scopus citations

Abstract

Rochalimaea (Bartonella) henselae is a fastidious, slowly growing, gram- negative bacillus that is an etiologic agent of bacillary angiomatosis, cat scratch disease, and related syndromes. Accumulation of direct microbiologic evidence of the relationship between the organism and the syndromes compatible with cat scratch disease has been hindered by the difficulties in the primary isolation of the organism from infected tissue specimens. A chemically defined liquid medium was developed to support the growth of Rochalimaea species to facilitate study of the organism. This medium was also used successfully to isolate R. henselae from clinical specimens from infected patients and a domestic cat. Recovery of R. henselae in this was more successful than when recovery was attempted on solid agar. This cell- free, extract-free, defined medium additionally supported the growth of Rochalimaea quintana and Afipia felis.

Original languageEnglish
Pages (from-to)742-744
Number of pages3
JournalJournal of Clinical Microbiology
Volume33
Issue number3
DOIs
StatePublished - 1995
Externally publishedYes

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