TY - JOUR
T1 - A Composite Recombinant Salivary Proteins Biomarker for Phlebotomus argentipes Provides a Surveillance Tool Postelimination of Visceral Leishmaniasis in India
AU - Iniguez, Eva
AU - Saha, Samiran
AU - Petrellis, Georgios
AU - Menenses, Claudio
AU - Herbert, Samantha
AU - Gonzalez-Rangel, Yvonne
AU - Rowland, Tobin
AU - Aronson, Naomi E.
AU - Rose, Clair
AU - Haines, Lee Rafuse
AU - Acosta-Serrano, Alvaro
AU - Serafim, Tiago D.
AU - Oliveira, Fabiano
AU - Srikantiah, Sridhar
AU - Bern, Caryn
AU - Valenzuela, Jesus G.
AU - Kamhawi, Shaden
N1 - Funding Information:
We thank the Protein Expression Laboratory at NCI-Frederick (Frederick, MD) for the expression of recombinant proteins and the Research Technologies Branch at National Institutes of Health, National Institute of Allergy and Infectious Diseases (NIH, NIAID) for analysis and identification of recombinant proteins by mass-spectrometry; and Michael P. Fey, PhD at the Biostatistics Research Branch at NIH, NIAID for his statistical input on this manuscript. Yonas Gebremicale at the Laboratory of Malaria and Vector Research, NIH, NIAID reared and provided the Anopheles albopictus and Aedes aegypti mosquitoes used for salivary gland dissections and immunoblotting. We thank Mara Short for the dissection of Anopheles albopictus salivary glands for the study. This work was supported by the NIH, NIAID Intramural Research Program; the Bill and Melinda Gates Foundation (grant number INV-008856/OPP1196454); and the Global Emerging Infections Surveillance Section, Armed Forces Health Surveillance Branch, Department of Defense (grant number PRoMIS ID P0024_17_HS).
Publisher Copyright:
© 2022 Oxford University Press. All rights reserved.
PY - 2022/11/15
Y1 - 2022/11/15
N2 - Incidence of visceral leishmaniasis (VL) in the Indian subcontinent (ISC) has declined by more than 95% since initiation of the elimination program in 2005. As the ISC transitions to the postelimination surveillance phase, an accurate measurement of human-vector contact is needed to assure long-term success. To develop this tool, we identified PagSP02 and PagSP06 from saliva of Phlebotomus argentipes, the vector of Leishmania donovani in the ISC, as immunodominant proteins in humans. We also established the absence of cross-reactivity with Phlebotomus papatasi saliva, the only other human-biting sand fly in the ISC. Importantly, by combining recombinant rPagSP02 and rPagSP06 we achieved greater antibody recognition and specificity than single salivary proteins. The receiver operating characteristics curve for rPagSP02 + rPagSP06 predicts exposure to Ph. argentipes bites with 90% specificity and 87% sensitivity compared to negative control sera (P >.0001). Overall, rPagSP02 + rPagSP06 provides an effective surveillance tool for monitoring vector control efforts after VL elimination.
AB - Incidence of visceral leishmaniasis (VL) in the Indian subcontinent (ISC) has declined by more than 95% since initiation of the elimination program in 2005. As the ISC transitions to the postelimination surveillance phase, an accurate measurement of human-vector contact is needed to assure long-term success. To develop this tool, we identified PagSP02 and PagSP06 from saliva of Phlebotomus argentipes, the vector of Leishmania donovani in the ISC, as immunodominant proteins in humans. We also established the absence of cross-reactivity with Phlebotomus papatasi saliva, the only other human-biting sand fly in the ISC. Importantly, by combining recombinant rPagSP02 and rPagSP06 we achieved greater antibody recognition and specificity than single salivary proteins. The receiver operating characteristics curve for rPagSP02 + rPagSP06 predicts exposure to Ph. argentipes bites with 90% specificity and 87% sensitivity compared to negative control sera (P >.0001). Overall, rPagSP02 + rPagSP06 provides an effective surveillance tool for monitoring vector control efforts after VL elimination.
KW - India
KW - Phlebotomus argentipes
KW - biomarker
KW - elimination campaign
KW - sand fly salivary proteins
KW - surveillance
KW - visceral leishmaniasis
UR - http://www.scopus.com/inward/record.url?scp=85141892120&partnerID=8YFLogxK
U2 - 10.1093/infdis/jiac354
DO - 10.1093/infdis/jiac354
M3 - Article
C2 - 36052609
AN - SCOPUS:85141892120
SN - 0022-1899
VL - 226
SP - 1842
EP - 1851
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 10
ER -