A comprehensive protein expression profile of extracellular matrix biomaterial derived from porcine urinary bladder

Helder Maral, Tania Ahmed, Stephen F. Badylak, Stephen Tottey, L. John R. Foster*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Aims: To generate a comprehensive profile of the protein composition of xenogeneic biomaterial, derived from porcine urinary bladder matrix (UBM). Materials & methods: Tunica layers and muscularis mucosa were removed from bladders using mechanical delamination. UBM was prepared using a solution of peracetic acid in ethanol, lyophilized then milled into powder. UBM biomaterial was subjected to tryptic digests and components separated using high-performance liquid chromatography with an ion trap mass spectrometer and identified through databases. Results: A repertoire of 129 proteins with neurotrophic, antiangiogenic and tumor-suppressive activities and those associated with tissue remodeling and wound repair were identified. Conclusion: This study provides the first insight into the complex nature of the UBM and how its application may be tailored for specific applications in regenerative medicine. We propose that the UBM be further investigated for reconstructive and regenerative remodeling of cardiac and dermal tissues, as well as peripheral nerves.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalRegenerative Medicine
Volume7
Issue number2
DOIs
StatePublished - Mar 2012
Externally publishedYes

Keywords

  • ECM
  • UBM
  • biomaterial
  • extracellular matrix
  • proteomics
  • scaffold
  • tissue engineering
  • tissue regeneration
  • urinary bladder matrix

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