A low-density antigen array for detection of diseaseassociated autoantibodies in human plasma

Richard I. Somiari, Rebecca Sutphen, Kutralanathan Renganathan, Stephen Russell, Elzbieta Pastwa, Stella A. Somiari*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Background/Aim: The ability to easily detect autoantibodies will help in the early diagnosis and treatment of certain diseases. Currently, available methods for autoantibody detection are time-consuming and cumbersome. The present study aimed to evaluate the performance of an easy-to-use antigen array developed for autoantibody detection. Materials and Methods: Plasma from 9 female donors diagnosed with ovarian cancer (test group) and 9 matched donors with no history of cancer (reference group) were screened and results were compared. Autoantibody levels ≥1.5-times the background were classified as positive. Results: A total of 29 autoantibodies were detected, out of which the autoantibody against osteoprotegerin was found to be significantly higher in the "test" group (p<0.001) while those against macrophage migration inhibitor factor, interleukin-2 and vascular endothelial growth factor were lower (p<0.05). Conclusion: The evaluated antigen array has potential as a simple method for determining the presence/absence of up to 90 disease-associated autoantibodies in a plasma specimen.

Original languageEnglish
Pages (from-to)13-20
Number of pages8
JournalCancer Genomics and Proteomics
Volume13
Issue number1
StatePublished - 1 Jan 2016
Externally publishedYes

Keywords

  • Antigene array
  • Autoantibody detection
  • Autoimmune disease
  • Ovarian cancer
  • Plasma

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