Abstract
Background/Aim: The ability to easily detect autoantibodies will help in the early diagnosis and treatment of certain diseases. Currently, available methods for autoantibody detection are time-consuming and cumbersome. The present study aimed to evaluate the performance of an easy-to-use antigen array developed for autoantibody detection. Materials and Methods: Plasma from 9 female donors diagnosed with ovarian cancer (test group) and 9 matched donors with no history of cancer (reference group) were screened and results were compared. Autoantibody levels ≥1.5-times the background were classified as positive. Results: A total of 29 autoantibodies were detected, out of which the autoantibody against osteoprotegerin was found to be significantly higher in the "test" group (p<0.001) while those against macrophage migration inhibitor factor, interleukin-2 and vascular endothelial growth factor were lower (p<0.05). Conclusion: The evaluated antigen array has potential as a simple method for determining the presence/absence of up to 90 disease-associated autoantibodies in a plasma specimen.
| Original language | English |
|---|---|
| Pages (from-to) | 13-20 |
| Number of pages | 8 |
| Journal | Cancer Genomics and Proteomics |
| Volume | 13 |
| Issue number | 1 |
| State | Published - 1 Jan 2016 |
| Externally published | Yes |
Keywords
- Antigene array
- Autoantibody detection
- Autoimmune disease
- Ovarian cancer
- Plasma