A malaria gametocytocidal assay using oxidoreduction indicator, alamarBlue

Takeshi Q. Tanaka; Kim C. Williamson

doi:10.1016/j.molbiopara.2011.02.005

A malaria gametocytocidal assay using oxidoreduction indicator, alamarBlue

Takeshi Q. Tanaka, Kim C. Williamson

Research output: Contribution to journal › Article › peer-review

83 Scopus citations

Abstract

Efforts to move from malaria control to eradication will require new approaches to block malaria transmission, such as the development of anti-malarial drugs with gametocytocidal activity. Here fluorescent oxidoreduction indicator alamarBlue is used to develop a screen for gametocyte viability. The fluorescent signal increases linearly with gametocyte number (R² = 0.99) and determination of the IC₅₀ of epoxomicin demonstrated the assay was reproducible and sensitive (IC₅₀ 2.16 ± 0.57 nM, Z′-factor 0.81 ± 0.01). Six anti-malarials were also tested and at 10 μM only primaquine and dihydroartemisinin (DHA) had gametocytocidal activity. This new assay provides an important tool to efficiently screen compounds for gametocytocidal activity.

Original language	English
Pages (from-to)	160-163
Number of pages	4
Journal	Molecular and Biochemical Parasitology
Volume	177
Issue number	2
DOIs	https://doi.org/10.1016/j.molbiopara.2011.02.005
State	Published - Jun 2011
Externally published	Yes

Keywords

AlamarBlue
Drug screening
Gametocyte
Plasmodium falciparum

Access to Document

10.1016/j.molbiopara.2011.02.005

Cite this

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title = "A malaria gametocytocidal assay using oxidoreduction indicator, alamarBlue",

abstract = "Efforts to move from malaria control to eradication will require new approaches to block malaria transmission, such as the development of anti-malarial drugs with gametocytocidal activity. Here fluorescent oxidoreduction indicator alamarBlue is used to develop a screen for gametocyte viability. The fluorescent signal increases linearly with gametocyte number (R2 = 0.99) and determination of the IC50 of epoxomicin demonstrated the assay was reproducible and sensitive (IC50 2.16 ± 0.57 nM, Z′-factor 0.81 ± 0.01). Six anti-malarials were also tested and at 10 μM only primaquine and dihydroartemisinin (DHA) had gametocytocidal activity. This new assay provides an important tool to efficiently screen compounds for gametocytocidal activity.",

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