TY - JOUR
T1 - A mechanism of resistance to HIV-1 entry
T2 - Inefficient interactions of CXCR4 with CD4 and gp120 in macrophages
AU - Dimitrov, Dimiter S.
AU - Norwood, David
AU - Stantchev, Tzanko S.
AU - Feng, Yanru
AU - Xiao, Xiaodong
AU - Broder, Christopher C.
PY - 1999/6/20
Y1 - 1999/6/20
N2 - To test the hypothesis that inefficient interactions of CXCR4 with CD4 and gp120 could affect HIV-1 entry, we incubated macrophages, monocytes, and lymphocytes with gp120 and coimmunoprecipitated CD4 by using anti-CXCR4 antibodies. CD4 was efficiently coimmunoprecipitated in lymphocytes and monocytes but not in macrophages. Overexpression of CD4 in macrophages resulted in detection of CD4-CXCR4 and gp120-CD4-CXCR4 complexes in parallel with the restoration of macrophage fusion susceptibility. These results suggest a mechanism of resistance to entry of some X4 HIV-1 strains into macrophages and a method for dissection of the initial stages of HIV entry.
AB - To test the hypothesis that inefficient interactions of CXCR4 with CD4 and gp120 could affect HIV-1 entry, we incubated macrophages, monocytes, and lymphocytes with gp120 and coimmunoprecipitated CD4 by using anti-CXCR4 antibodies. CD4 was efficiently coimmunoprecipitated in lymphocytes and monocytes but not in macrophages. Overexpression of CD4 in macrophages resulted in detection of CD4-CXCR4 and gp120-CD4-CXCR4 complexes in parallel with the restoration of macrophage fusion susceptibility. These results suggest a mechanism of resistance to entry of some X4 HIV-1 strains into macrophages and a method for dissection of the initial stages of HIV entry.
KW - CD4
KW - Chemokine receptors
KW - Coreceptors
KW - CXCR4
KW - Envelope glycoprotein
KW - HIV-1
KW - Membrane fusion
UR - http://www.scopus.com/inward/record.url?scp=0033587321&partnerID=8YFLogxK
U2 - 10.1006/viro.1999.9747
DO - 10.1006/viro.1999.9747
M3 - Article
C2 - 10364484
AN - SCOPUS:0033587321
SN - 0042-6822
VL - 259
SP - 1
EP - 6
JO - Virology
JF - Virology
IS - 1
ER -