A mechanism of resistance to HIV-1 entry: Inefficient interactions of CXCR4 with CD4 and gp120 in macrophages

Dimiter S. Dimitrov; David Norwood; Tzanko S. Stantchev; Yanru Feng; Xiaodong Xiao; Christopher C. Broder

doi:10.1006/viro.1999.9747

A mechanism of resistance to HIV-1 entry: Inefficient interactions of CXCR4 with CD4 and gp120 in macrophages

Dimiter S. Dimitrov^*, David Norwood, Tzanko S. Stantchev, Yanru Feng, Xiaodong Xiao, Christopher C. Broder

^*Corresponding author for this work

Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

To test the hypothesis that inefficient interactions of CXCR4 with CD4 and gp120 could affect HIV-1 entry, we incubated macrophages, monocytes, and lymphocytes with gp120 and coimmunoprecipitated CD4 by using anti-CXCR4 antibodies. CD4 was efficiently coimmunoprecipitated in lymphocytes and monocytes but not in macrophages. Overexpression of CD4 in macrophages resulted in detection of CD4-CXCR4 and gp120-CD4-CXCR4 complexes in parallel with the restoration of macrophage fusion susceptibility. These results suggest a mechanism of resistance to entry of some X4 HIV-1 strains into macrophages and a method for dissection of the initial stages of HIV entry.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Virology
Volume	259
Issue number	1
DOIs	https://doi.org/10.1006/viro.1999.9747
State	Published - 20 Jun 1999

Keywords

CD4
CXCR4
Chemokine receptors
Coreceptors
Envelope glycoprotein
HIV-1
Membrane fusion

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10.1006/viro.1999.9747

Cite this

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abstract = "To test the hypothesis that inefficient interactions of CXCR4 with CD4 and gp120 could affect HIV-1 entry, we incubated macrophages, monocytes, and lymphocytes with gp120 and coimmunoprecipitated CD4 by using anti-CXCR4 antibodies. CD4 was efficiently coimmunoprecipitated in lymphocytes and monocytes but not in macrophages. Overexpression of CD4 in macrophages resulted in detection of CD4-CXCR4 and gp120-CD4-CXCR4 complexes in parallel with the restoration of macrophage fusion susceptibility. These results suggest a mechanism of resistance to entry of some X4 HIV-1 strains into macrophages and a method for dissection of the initial stages of HIV entry.",

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AU - Norwood, David

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AU - Xiao, Xiaodong

AU - Broder, Christopher C.

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AB - To test the hypothesis that inefficient interactions of CXCR4 with CD4 and gp120 could affect HIV-1 entry, we incubated macrophages, monocytes, and lymphocytes with gp120 and coimmunoprecipitated CD4 by using anti-CXCR4 antibodies. CD4 was efficiently coimmunoprecipitated in lymphocytes and monocytes but not in macrophages. Overexpression of CD4 in macrophages resulted in detection of CD4-CXCR4 and gp120-CD4-CXCR4 complexes in parallel with the restoration of macrophage fusion susceptibility. These results suggest a mechanism of resistance to entry of some X4 HIV-1 strains into macrophages and a method for dissection of the initial stages of HIV entry.

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