A murine model of volumetric muscle loss and a regenerative medicine approach for tissue replacement

Brian M. Sicari, Vineet Agrawal, Bernard F. Siu, Christopher J. Medberry, Christopher L. Dearth, Neill J. Turner, Stephen F. Badylak*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

130 Scopus citations

Abstract

Volumetric muscle loss (VML) resulting from traumatic accidents, tumor ablation, or degenerative disease is associated with limited treatment options and high morbidity. The lack of a reliable and reproducible animal model of VML has hindered the development of effective therapeutic strategies. The present study describes a critical-sized excisional defect within the mouse quadriceps muscle that results in an irrecoverable volumetric defect. This model of VML was used to evaluate the efficacy of a surgically placed inductive biologic scaffold material composed of porcine small intestinal submucosa-extracellular matrix (SIS-ECM). The targeted placement of an SIS-ECM scaffold within the defect was associated with constructive tissue remodeling including the formation of site-appropriate skeletal muscle tissue. The present study provides a reproducible animal model with which to study VML and shows the therapeutic potential of a bioscaffold-based regenerative medicine approach to VML.

Original languageEnglish
Pages (from-to)1941-1948
Number of pages8
JournalTissue Engineering - Part A.
Volume18
Issue number19-20
DOIs
StatePublished - 1 Oct 2012
Externally publishedYes

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