TY - JOUR
T1 - A murine model of volumetric muscle loss and a regenerative medicine approach for tissue replacement
AU - Sicari, Brian M.
AU - Agrawal, Vineet
AU - Siu, Bernard F.
AU - Medberry, Christopher J.
AU - Dearth, Christopher L.
AU - Turner, Neill J.
AU - Badylak, Stephen F.
PY - 2012/10/1
Y1 - 2012/10/1
N2 - Volumetric muscle loss (VML) resulting from traumatic accidents, tumor ablation, or degenerative disease is associated with limited treatment options and high morbidity. The lack of a reliable and reproducible animal model of VML has hindered the development of effective therapeutic strategies. The present study describes a critical-sized excisional defect within the mouse quadriceps muscle that results in an irrecoverable volumetric defect. This model of VML was used to evaluate the efficacy of a surgically placed inductive biologic scaffold material composed of porcine small intestinal submucosa-extracellular matrix (SIS-ECM). The targeted placement of an SIS-ECM scaffold within the defect was associated with constructive tissue remodeling including the formation of site-appropriate skeletal muscle tissue. The present study provides a reproducible animal model with which to study VML and shows the therapeutic potential of a bioscaffold-based regenerative medicine approach to VML.
AB - Volumetric muscle loss (VML) resulting from traumatic accidents, tumor ablation, or degenerative disease is associated with limited treatment options and high morbidity. The lack of a reliable and reproducible animal model of VML has hindered the development of effective therapeutic strategies. The present study describes a critical-sized excisional defect within the mouse quadriceps muscle that results in an irrecoverable volumetric defect. This model of VML was used to evaluate the efficacy of a surgically placed inductive biologic scaffold material composed of porcine small intestinal submucosa-extracellular matrix (SIS-ECM). The targeted placement of an SIS-ECM scaffold within the defect was associated with constructive tissue remodeling including the formation of site-appropriate skeletal muscle tissue. The present study provides a reproducible animal model with which to study VML and shows the therapeutic potential of a bioscaffold-based regenerative medicine approach to VML.
UR - http://www.scopus.com/inward/record.url?scp=84867011448&partnerID=8YFLogxK
U2 - 10.1089/ten.tea.2012.0475
DO - 10.1089/ten.tea.2012.0475
M3 - Article
C2 - 22906411
AN - SCOPUS:84867011448
SN - 1937-3341
VL - 18
SP - 1941
EP - 1948
JO - Tissue Engineering - Part A.
JF - Tissue Engineering - Part A.
IS - 19-20
ER -