TY - JOUR
T1 - A neutralization test for specific detection of Nipah virus antibodies using pseudotyped vesicular stomatitis virus expressing green fluorescent protein
AU - Kaku, Yoshihiro
AU - Noguchi, Akira
AU - Marsh, Glenn A.
AU - McEachern, Jennifer A.
AU - Okutani, Akiko
AU - Hotta, Kozue
AU - Bazartseren, Boldbaatar
AU - Fukushi, Shuetsu
AU - Broder, Christopher C.
AU - Yamada, Akio
AU - Inoue, Satoshi
AU - Wang, Lin Fa
PY - 2009/9
Y1 - 2009/9
N2 - Nipah virus (NiV) is a new zoonotic paramyxovirus that emerged in 1998 and is now classified in the genus Henipavirus along with the closely related Hendra virus (HeV). NiV is highly pathogenic in several vertebrate species including humans, and the lack of available vaccines or specific treatment restricts it to biosafety level 4 (BSL4) containment. A serum neutralization test was developed for measuring NiV neutralizing antibodies under BSL2 conditions using a recombinant vesicular stomatitis virus (VSV) expressing green fluorescent protein (GFP) and bearing the F and G proteins of NiV (VSV-NiV-GFP). The neutralization titers were obtained by counting GFP-expressing cells or by measuring fluorescence. The performance of this new assay was compared against the conventional test using live NiV with panels of sera from several mammalian species, including sera from NiV outbreaks, experimental infections, as well as HeV-specific sera. The results obtained with the VSV-NiV-GFP based test correlated with those obtained using live NiV. Using a 50% reduction in VSV-NiV-GFP infected cells as the cut-off for neutralization, this new assay demonstrated its potential as an effective tool for detecting NiV neutralizing antibodies under BSL2 containment with greater speed, sensitivity and safety as compared to the conventional NiV serum neutralization test.
AB - Nipah virus (NiV) is a new zoonotic paramyxovirus that emerged in 1998 and is now classified in the genus Henipavirus along with the closely related Hendra virus (HeV). NiV is highly pathogenic in several vertebrate species including humans, and the lack of available vaccines or specific treatment restricts it to biosafety level 4 (BSL4) containment. A serum neutralization test was developed for measuring NiV neutralizing antibodies under BSL2 conditions using a recombinant vesicular stomatitis virus (VSV) expressing green fluorescent protein (GFP) and bearing the F and G proteins of NiV (VSV-NiV-GFP). The neutralization titers were obtained by counting GFP-expressing cells or by measuring fluorescence. The performance of this new assay was compared against the conventional test using live NiV with panels of sera from several mammalian species, including sera from NiV outbreaks, experimental infections, as well as HeV-specific sera. The results obtained with the VSV-NiV-GFP based test correlated with those obtained using live NiV. Using a 50% reduction in VSV-NiV-GFP infected cells as the cut-off for neutralization, this new assay demonstrated its potential as an effective tool for detecting NiV neutralizing antibodies under BSL2 containment with greater speed, sensitivity and safety as compared to the conventional NiV serum neutralization test.
KW - Biosafety
KW - Diagnosis
KW - Henipavirus
KW - Neutralization
KW - Nipah
KW - Pseudotype
UR - http://www.scopus.com/inward/record.url?scp=67549139880&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2009.04.037
DO - 10.1016/j.jviromet.2009.04.037
M3 - Article
C2 - 19433112
AN - SCOPUS:67549139880
SN - 0166-0934
VL - 160
SP - 7
EP - 13
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1-2
ER -