A novel high-throughput screening system to evaluate the behavioral response of adult mosquitoes to chemicals

John P. Grieco*, Nicole L. Achee, Michael R. Sardelis, Kamlesh R. Chauhan, Donald R. Roberts

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

A modular and novel assay system for rapid mass screening of chemical compounds for contact irritant and spatial repellent actions against adult mosquitoes is described. The responses of Aedes aegypti to various concentrations of 3 topical repellents, deet, Bayrepel®, and SS220, were evaluated. At treatment concentrations ≥25 nmol/cm2 of SS220, mosquitoes exhibited significant contact irritant (escape) and spatial repellent (movement away from the chemical source) responses, whereas, a 10-fold increase in the treatment concentration of deet and Bayrepel was required to produce similar responses. The novel bioassay system detected contact irritancy and spatial repellency activity with reproducible results and provided baseline data for determining minimum effective concentrations for other chemicals. The system is compact in size, easy to decontaminate, and requires only a minute quantity of chemical compound.

Original languageEnglish
Pages (from-to)404-411
Number of pages8
JournalJournal of the American Mosquito Control Association
Volume21
Issue number4
DOIs
StatePublished - Dec 2005
Externally publishedYes

Keywords

  • Assay
  • Behavior
  • Contact irritancy
  • Mosquitoes
  • Screening
  • Spatial repellency

Fingerprint

Dive into the research topics of 'A novel high-throughput screening system to evaluate the behavioral response of adult mosquitoes to chemicals'. Together they form a unique fingerprint.

Cite this