A novel high-throughput screening system to evaluate the behavioral response of adult mosquitoes to chemicals

John P. Grieco; Nicole L. Achee; Michael R. Sardelis; Kamlesh R. Chauhan; Donald R. Roberts

doi:10.2987/8756-971X(2006)21[404:ANHSST]2.0.CO;2

A novel high-throughput screening system to evaluate the behavioral response of adult mosquitoes to chemicals

John P. Grieco^*, Nicole L. Achee, Michael R. Sardelis, Kamlesh R. Chauhan, Donald R. Roberts

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

98 Scopus citations

Abstract

A modular and novel assay system for rapid mass screening of chemical compounds for contact irritant and spatial repellent actions against adult mosquitoes is described. The responses of Aedes aegypti to various concentrations of 3 topical repellents, deet, Bayrepel®, and SS220, were evaluated. At treatment concentrations ≥25 nmol/cm² of SS220, mosquitoes exhibited significant contact irritant (escape) and spatial repellent (movement away from the chemical source) responses, whereas, a 10-fold increase in the treatment concentration of deet and Bayrepel was required to produce similar responses. The novel bioassay system detected contact irritancy and spatial repellency activity with reproducible results and provided baseline data for determining minimum effective concentrations for other chemicals. The system is compact in size, easy to decontaminate, and requires only a minute quantity of chemical compound.

Original language	English
Pages (from-to)	404-411
Number of pages	8
Journal	Journal of the American Mosquito Control Association
Volume	21
Issue number	4
DOIs	https://doi.org/10.2987/8756-971X(2006)21[404:ANHSST]2.0.CO;2
State	Published - Dec 2005
Externally published	Yes

Keywords

Assay
Behavior
Contact irritancy
Mosquitoes
Screening
Spatial repellency

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10.2987/8756-971X(2006)21[404:ANHSST]2.0.CO;2

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title = "A novel high-throughput screening system to evaluate the behavioral response of adult mosquitoes to chemicals",

abstract = "A modular and novel assay system for rapid mass screening of chemical compounds for contact irritant and spatial repellent actions against adult mosquitoes is described. The responses of Aedes aegypti to various concentrations of 3 topical repellents, deet, Bayrepel{\textregistered}, and SS220, were evaluated. At treatment concentrations ≥25 nmol/cm2 of SS220, mosquitoes exhibited significant contact irritant (escape) and spatial repellent (movement away from the chemical source) responses, whereas, a 10-fold increase in the treatment concentration of deet and Bayrepel was required to produce similar responses. The novel bioassay system detected contact irritancy and spatial repellency activity with reproducible results and provided baseline data for determining minimum effective concentrations for other chemicals. The system is compact in size, easy to decontaminate, and requires only a minute quantity of chemical compound.",

keywords = "Assay, Behavior, Contact irritancy, Mosquitoes, Screening, Spatial repellency",

author = "Grieco, {John P.} and Achee, {Nicole L.} and Sardelis, {Michael R.} and Chauhan, {Kamlesh R.} and Roberts, {Donald R.}",

year = "2005",

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doi = "10.2987/8756-971X(2006)21[404:ANHSST]2.0.CO;2",

language = "English",

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pages = "404--411",

journal = "Journal of the American Mosquito Control Association",

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AU - Achee, Nicole L.

AU - Sardelis, Michael R.

AU - Chauhan, Kamlesh R.

AU - Roberts, Donald R.

PY - 2005/12

Y1 - 2005/12

N2 - A modular and novel assay system for rapid mass screening of chemical compounds for contact irritant and spatial repellent actions against adult mosquitoes is described. The responses of Aedes aegypti to various concentrations of 3 topical repellents, deet, Bayrepel®, and SS220, were evaluated. At treatment concentrations ≥25 nmol/cm2 of SS220, mosquitoes exhibited significant contact irritant (escape) and spatial repellent (movement away from the chemical source) responses, whereas, a 10-fold increase in the treatment concentration of deet and Bayrepel was required to produce similar responses. The novel bioassay system detected contact irritancy and spatial repellency activity with reproducible results and provided baseline data for determining minimum effective concentrations for other chemicals. The system is compact in size, easy to decontaminate, and requires only a minute quantity of chemical compound.

AB - A modular and novel assay system for rapid mass screening of chemical compounds for contact irritant and spatial repellent actions against adult mosquitoes is described. The responses of Aedes aegypti to various concentrations of 3 topical repellents, deet, Bayrepel®, and SS220, were evaluated. At treatment concentrations ≥25 nmol/cm2 of SS220, mosquitoes exhibited significant contact irritant (escape) and spatial repellent (movement away from the chemical source) responses, whereas, a 10-fold increase in the treatment concentration of deet and Bayrepel was required to produce similar responses. The novel bioassay system detected contact irritancy and spatial repellency activity with reproducible results and provided baseline data for determining minimum effective concentrations for other chemicals. The system is compact in size, easy to decontaminate, and requires only a minute quantity of chemical compound.

KW - Assay

KW - Behavior

KW - Contact irritancy

KW - Mosquitoes

KW - Screening

KW - Spatial repellency

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A novel high-throughput screening system to evaluate the behavioral response of adult mosquitoes to chemicals

Abstract

Keywords

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