A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum

D. V. Agoston; J. Fahrenkrug; J. D. Mikkelsen; V. P. Whittaker

doi:10.1016/0196-9781(89)90144-7

A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum

D. V. Agoston, J. Fahrenkrug, J. D. Mikkelsen, V. P. Whittaker^*

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.

Original language	English
Pages (from-to)	571-573
Number of pages	3
Journal	Peptides
Volume	10
Issue number	3
DOIs	https://doi.org/10.1016/0196-9781(89)90144-7
State	Published - 1989

Keywords

Copackaging
Myenteric neurones
PHI
VIP

Access to Document

10.1016/0196-9781(89)90144-7

Cite this

@article{2358602a19cd456f8886509323e24555,

title = "A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum",

abstract = "When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.",

keywords = "Copackaging, Myenteric neurones, PHI, VIP",

author = "Agoston, \{D. V.\} and J. Fahrenkrug and Mikkelsen, \{J. D.\} and Whittaker, \{V. P.\}",

year = "1989",

doi = "10.1016/0196-9781(89)90144-7",

language = "English",

volume = "10",

pages = "571--573",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum

AU - Agoston, D. V.

AU - Fahrenkrug, J.

AU - Mikkelsen, J. D.

AU - Whittaker, V. P.

PY - 1989

Y1 - 1989

N2 - When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.

AB - When cytoplasmic extracts of the myenteric plexus of guinea pig ileum are submitted to centrifugal density gradient separation in a zonal rotor, conditions which separate storage particles containing substance P, somatostatin and VIP from each other, PHI copurifies with VIP. The two immunoreactivities cannot be separated by particle exclusion chromatography, which depends on size rather than density. It is concluded that the posttranslational cleavage of the propeptide or precursor to PHI and VIP occurs after packaging into these storage particles.

KW - Copackaging

KW - Myenteric neurones

KW - PHI

KW - VIP

UR - http://www.scopus.com/inward/record.url?scp=0024364030&partnerID=8YFLogxK

U2 - 10.1016/0196-9781(89)90144-7

DO - 10.1016/0196-9781(89)90144-7

M3 - Article

C2 - 2780416

AN - SCOPUS:0024364030

SN - 0196-9781

VL - 10

SP - 571

EP - 573

JO - Peptides

JF - Peptides

IS - 3

ER -

A peptide with N-terminal histidine and C-terminal isoleucine amide (PHI) and vasoactive intestinal peptide (VIP) are copackaged in myenteric neurones of the guinea pig ileum

Abstract

Keywords

Access to Document

Fingerprint

Cite this