TY - JOUR
T1 - A Perivascular Origin for Mesenchymal Stem Cells in Multiple Human Organs
AU - Crisan, Mihaela
AU - Yap, Solomon
AU - Casteilla, Louis
AU - Chen, Chien Wen
AU - Corselli, Mirko
AU - Park, Tea Soon
AU - Andriolo, Gabriella
AU - Sun, Bin
AU - Zheng, Bo
AU - Zhang, Li
AU - Norotte, Cyrille
AU - Teng, Pang Ning
AU - Traas, Jeremy
AU - Schugar, Rebecca
AU - Deasy, Bridget M.
AU - Badylak, Stephen
AU - Buhring, Hans Jörg
AU - Giacobino, Jean Paul
AU - Lazzari, Lorenza
AU - Huard, Johnny
AU - Péault, Bruno
N1 - Funding Information:
Our thanks are due to Allison Logar for assistance with flow cytometry; Mitra Lavasani for help with in vivo assays; and Dr. Rita Bottino, Dr. Burhan Gharaibeh, Dr. Peter Rubin, and Lindsay Mock for procurement of human tissues. Dr. Simon Watkins assisted with confocal microscopy, Roseanne Perry helped with manuscript preparation, and David Humiston proofread this report. This work was supported in part by grants from the Department of Defense, Commonwealth of Pennsylvania, Children's Hospital of Pittsburgh, University of Pittsburgh Cancer Institute, and National Institutes of Health (RO1 #AR49684-01 and R21 #HL083057-01A2). M.C. was supported by Banca San Paolo of Turin, Italy.
PY - 2008/9/11
Y1 - 2008/9/11
N2 - Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-Rβ expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells.
AB - Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-Rβ expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells.
KW - STEMCELL
UR - http://www.scopus.com/inward/record.url?scp=50849139576&partnerID=8YFLogxK
U2 - 10.1016/j.stem.2008.07.003
DO - 10.1016/j.stem.2008.07.003
M3 - Article
C2 - 18786417
AN - SCOPUS:50849139576
SN - 1934-5909
VL - 3
SP - 301
EP - 313
JO - Cell Stem Cell
JF - Cell Stem Cell
IS - 3
ER -