A processed metabolite of luteinizing hormone-releasing hormone has proliferative effects in endometrial cells

Kathryn Walters; Yue Pui Chin; T John Wu

doi:10.1016/j.ajog.2006.07.054

A processed metabolite of luteinizing hormone-releasing hormone has proliferative effects in endometrial cells

Kathryn Walters, Yue Pui Chin, T John Wu

Gynecologic Surgery and Obstetrics

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

OBJECTIVE: The purpose of this study is to determine the possible role of the processed peptide of LHRH, LHRH-(1-5), in regulating growth of endometrial cancer cells.

STUDY DESIGN: An endometrial cancer cell line, the Ishikawa cell line, was cultured under standard conditions and treated in a dose-dependent manner with 1 of 2 hormones, LHRH and LHRH-(1-5) to determine the ability of these peptides to regulate cellular growth. A tetrazolium-based assay was used to determine the effect these peptides have on cell proliferation. Furthermore, enzyme-linked immunosorbent assay (ELISA)-based assays were used to determine the expression of caspase-3/7 and pERK-1/2. Statistical analyses were conducted using an analysis of variance followed by Fisher LSD as the post-hoc test.

RESULTS: The results show that LHRH is anti-proliferative whereas LHRH-(1-5) is proliferative on the cells. Furthermore, LHRH-(1-5) decreased caspase-3/7 and pERK1/2 expression.

CONCLUSION: This is the first time LHRH-(1-5) is shown to have proliferative effects on cells.

Original language	English
Pages (from-to)	33.e1-5
Journal	American Journal of Obstetrics and Gynecology
Volume	196
Issue number	1
DOIs	https://doi.org/10.1016/j.ajog.2006.07.054
State	Published - Jan 2007

Keywords

Cell Line, Tumor
Cell Proliferation/drug effects
Dose-Response Relationship, Drug
Endometrium/cytology
Female
Gonadotropin-Releasing Hormone/metabolism
Humans
Peptide Fragments/pharmacology
Tumor Cells, Cultured

Access to Document

10.1016/j.ajog.2006.07.054

Cite this

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title = "A processed metabolite of luteinizing hormone-releasing hormone has proliferative effects in endometrial cells",

abstract = "OBJECTIVE: The purpose of this study is to determine the possible role of the processed peptide of LHRH, LHRH-(1-5), in regulating growth of endometrial cancer cells.STUDY DESIGN: An endometrial cancer cell line, the Ishikawa cell line, was cultured under standard conditions and treated in a dose-dependent manner with 1 of 2 hormones, LHRH and LHRH-(1-5) to determine the ability of these peptides to regulate cellular growth. A tetrazolium-based assay was used to determine the effect these peptides have on cell proliferation. Furthermore, enzyme-linked immunosorbent assay (ELISA)-based assays were used to determine the expression of caspase-3/7 and pERK-1/2. Statistical analyses were conducted using an analysis of variance followed by Fisher LSD as the post-hoc test.RESULTS: The results show that LHRH is anti-proliferative whereas LHRH-(1-5) is proliferative on the cells. Furthermore, LHRH-(1-5) decreased caspase-3/7 and pERK1/2 expression.CONCLUSION: This is the first time LHRH-(1-5) is shown to have proliferative effects on cells.",

keywords = "Cell Line, Tumor, Cell Proliferation/drug effects, Dose-Response Relationship, Drug, Endometrium/cytology, Female, Gonadotropin-Releasing Hormone/metabolism, Humans, Peptide Fragments/pharmacology, Tumor Cells, Cultured",

author = "Kathryn Walters and Chin, {Yue Pui} and Wu, {T John}",

year = "2007",

month = jan,

doi = "10.1016/j.ajog.2006.07.054",

language = "English",

volume = "196",

pages = "33.e1--5",

journal = "American Journal of Obstetrics and Gynecology",

issn = "0002-9378",

publisher = "Elsevier Inc.",

number = "1",

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T1 - A processed metabolite of luteinizing hormone-releasing hormone has proliferative effects in endometrial cells

AU - Walters, Kathryn

AU - Chin, Yue Pui

AU - Wu, T John

PY - 2007/1

Y1 - 2007/1

N2 - OBJECTIVE: The purpose of this study is to determine the possible role of the processed peptide of LHRH, LHRH-(1-5), in regulating growth of endometrial cancer cells.STUDY DESIGN: An endometrial cancer cell line, the Ishikawa cell line, was cultured under standard conditions and treated in a dose-dependent manner with 1 of 2 hormones, LHRH and LHRH-(1-5) to determine the ability of these peptides to regulate cellular growth. A tetrazolium-based assay was used to determine the effect these peptides have on cell proliferation. Furthermore, enzyme-linked immunosorbent assay (ELISA)-based assays were used to determine the expression of caspase-3/7 and pERK-1/2. Statistical analyses were conducted using an analysis of variance followed by Fisher LSD as the post-hoc test.RESULTS: The results show that LHRH is anti-proliferative whereas LHRH-(1-5) is proliferative on the cells. Furthermore, LHRH-(1-5) decreased caspase-3/7 and pERK1/2 expression.CONCLUSION: This is the first time LHRH-(1-5) is shown to have proliferative effects on cells.

AB - OBJECTIVE: The purpose of this study is to determine the possible role of the processed peptide of LHRH, LHRH-(1-5), in regulating growth of endometrial cancer cells.STUDY DESIGN: An endometrial cancer cell line, the Ishikawa cell line, was cultured under standard conditions and treated in a dose-dependent manner with 1 of 2 hormones, LHRH and LHRH-(1-5) to determine the ability of these peptides to regulate cellular growth. A tetrazolium-based assay was used to determine the effect these peptides have on cell proliferation. Furthermore, enzyme-linked immunosorbent assay (ELISA)-based assays were used to determine the expression of caspase-3/7 and pERK-1/2. Statistical analyses were conducted using an analysis of variance followed by Fisher LSD as the post-hoc test.RESULTS: The results show that LHRH is anti-proliferative whereas LHRH-(1-5) is proliferative on the cells. Furthermore, LHRH-(1-5) decreased caspase-3/7 and pERK1/2 expression.CONCLUSION: This is the first time LHRH-(1-5) is shown to have proliferative effects on cells.

KW - Cell Line, Tumor

KW - Cell Proliferation/drug effects

KW - Dose-Response Relationship, Drug

KW - Endometrium/cytology

KW - Female

KW - Gonadotropin-Releasing Hormone/metabolism

KW - Humans

KW - Peptide Fragments/pharmacology

KW - Tumor Cells, Cultured

U2 - 10.1016/j.ajog.2006.07.054

DO - 10.1016/j.ajog.2006.07.054

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SN - 0002-9378

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SP - 33.e1-5

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

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