A quantitative approach to target and identify cell surface marker proteins

Van M. Hoang; Thomas P. Conrads; Timothy D. Veenstra; Robert J. Fisher

A quantitative approach to target and identify cell surface marker proteins

Van M. Hoang^*, Thomas P. Conrads, Timothy D. Veenstra, Robert J. Fisher

^*Corresponding author for this work

Research output: Contribution to conference › Paper › peer-review

Abstract

A method was developed to quantitatively and selectively identify cell surface proteins that would be useful as markers for differentiated or diseased cells. The method utilized the matrix-assisted laser desorption ionization time-of-flight time-of-flight (MALDI-TOF-TOF) mass spectrometry for the analysis of the proteins. Methyl iodide was used in conjunction with Sulfo-NHS-SS-biotin for relative quantification of protein expression. the MALDI-TOF analysis after affinity isolation of the biotinylated peptides and reduction of the linker region showed successful labeling of the protein.

Original language	English
Pages	211-212
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2 Jun 2002 → 6 Jun 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	2/06/02 → 6/06/02

Cite this

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title = "A quantitative approach to target and identify cell surface marker proteins",

abstract = "A method was developed to quantitatively and selectively identify cell surface proteins that would be useful as markers for differentiated or diseased cells. The method utilized the matrix-assisted laser desorption ionization time-of-flight time-of-flight (MALDI-TOF-TOF) mass spectrometry for the analysis of the proteins. Methyl iodide was used in conjunction with Sulfo-NHS-SS-biotin for relative quantification of protein expression. the MALDI-TOF analysis after affinity isolation of the biotinylated peptides and reduction of the linker region showed successful labeling of the protein.",

author = "Hoang, {Van M.} and Conrads, {Thomas P.} and Veenstra, {Timothy D.} and Fisher, {Robert J.}",

year = "2002",

language = "English",

pages = "211--212",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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TY - CONF

T1 - A quantitative approach to target and identify cell surface marker proteins

AU - Hoang, Van M.

AU - Conrads, Thomas P.

AU - Veenstra, Timothy D.

AU - Fisher, Robert J.

PY - 2002

Y1 - 2002

N2 - A method was developed to quantitatively and selectively identify cell surface proteins that would be useful as markers for differentiated or diseased cells. The method utilized the matrix-assisted laser desorption ionization time-of-flight time-of-flight (MALDI-TOF-TOF) mass spectrometry for the analysis of the proteins. Methyl iodide was used in conjunction with Sulfo-NHS-SS-biotin for relative quantification of protein expression. the MALDI-TOF analysis after affinity isolation of the biotinylated peptides and reduction of the linker region showed successful labeling of the protein.

AB - A method was developed to quantitatively and selectively identify cell surface proteins that would be useful as markers for differentiated or diseased cells. The method utilized the matrix-assisted laser desorption ionization time-of-flight time-of-flight (MALDI-TOF-TOF) mass spectrometry for the analysis of the proteins. Methyl iodide was used in conjunction with Sulfo-NHS-SS-biotin for relative quantification of protein expression. the MALDI-TOF analysis after affinity isolation of the biotinylated peptides and reduction of the linker region showed successful labeling of the protein.

UR - http://www.scopus.com/inward/record.url?scp=2442641841&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442641841

SP - 211

EP - 212

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

A quantitative approach to target and identify cell surface marker proteins

Abstract

Conference

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