A SARS-CoV-2 Spike Ferritin Nanoparticle Vaccine Is Protective and Promotes a Strong Immunological Response in the Cynomolgus Macaque Coronavirus Disease 2019 (COVID-19) Model

Sara C. Johnston; Keersten M. Ricks; Ines Lakhal-Naouar; Alexandra Jay; Caroline Subra; Jo Lynne Raymond; Hannah A.D. King; Franco Rossi; Tamara L. Clements; David Fetterer; Samantha Tostenson; Camila Macedo Cincotta; Holly R. Hack; Caitlin Kuklis; Sandrine Soman; Jocelyn King; Kristina K. Peachman; Dohoon Kim; Wei Hung Chen; Rajeshwer S. Sankhala; Elizabeth J. Martinez; Agnes Hajduczki; William C. Chang; Misook Choe; Paul V. Thomas; Caroline E. Peterson; Alexander Anderson; Isabella Swafford; Jeffrey R. Currier; Dominic Paquin-Proulx; Linda L. Jagodzinski; Gary R. Matyas; Mangala Rao; Gregory D. Gromowski; Sheila A. Peel; Lauren White; Jeffrey M. Smith; Jay W. Hooper; Nelson L. Michael; Kayvon Modjarrad; M. Gordon Joyce; Aysegul Nalca; Diane L. Bolton; Margaret L.M. Pitt

doi:10.3390/vaccines10050717

A SARS-CoV-2 Spike Ferritin Nanoparticle Vaccine Is Protective and Promotes a Strong Immunological Response in the Cynomolgus Macaque Coronavirus Disease 2019 (COVID-19) Model

Sara C. Johnston^*, Keersten M. Ricks, Ines Lakhal-Naouar, Alexandra Jay, Caroline Subra, Jo Lynne Raymond, Hannah A.D. King, Franco Rossi, Tamara L. Clements, David Fetterer, Samantha Tostenson, Camila Macedo Cincotta, Holly R. Hack, Caitlin Kuklis, Sandrine Soman, Jocelyn King, Kristina K. Peachman, Dohoon Kim, Wei Hung Chen, Rajeshwer S. SankhalaElizabeth J. Martinez, Agnes Hajduczki, William C. Chang, Misook Choe, Paul V. Thomas, Caroline E. Peterson, Alexander Anderson, Isabella Swafford, Jeffrey R. Currier, Dominic Paquin-Proulx, Linda L. Jagodzinski, Gary R. Matyas, Mangala Rao, Gregory D. Gromowski, Sheila A. Peel, Lauren White, Jeffrey M. Smith, Jay W. Hooper, Nelson L. Michael, Kayvon Modjarrad, M. Gordon Joyce, Aysegul Nalca, Diane L. Bolton, Margaret L.M. Pitt

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The COVID-19 pandemic has had a staggering impact on social, economic, and public health systems worldwide. Vaccine development and mobilization against SARS-CoV-2 (the etio-logic agent of COVID-19) has been rapid. However, novel strategies are still necessary to slow the pandemic, and this includes new approaches to vaccine development and/or delivery that will improve vaccination compliance and demonstrate efficacy against emerging variants. Here, we report on the immunogenicity and efficacy of a SARS-CoV-2 vaccine comprising stabilized, pre-fusion spike protein trimers displayed on a ferritin nanoparticle (SpFN) adjuvanted with either conven-tional aluminum hydroxide or the Army Liposomal Formulation QS-21 (ALFQ) in a cynomolgus macaque COVID-19 model. Vaccination resulted in robust cell-mediated and humoral responses and a significant reduction in lung lesions following SARS-CoV-2 infection. The strength of the immune response suggests that dose sparing through reduced or single dosing in primates may be possible with this vaccine. Overall, the data support further evaluation of SpFN as a SARS-CoV-2 protein-based vaccine candidate with attention to fractional dosing and schedule optimization.

Original language	English
Article number	717
Journal	Vaccines
Volume	10
Issue number	5
DOIs	https://doi.org/10.3390/vaccines10050717
State	Published - May 2022
Externally published	Yes

Keywords

Army Liposomal Formulation QS-21
COVID-19
SARS-CoV-2
SpFN
aluminum hy-droxide
ferritin nanoparticle
primate
vaccine

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10.3390/vaccines10050717

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Johnston, S. C., Ricks, K. M., Lakhal-Naouar, I., Jay, A., Subra, C., Raymond, J. L., King, H. A. D., Rossi, F., Clements, T. L., Fetterer, D., Tostenson, S., Cincotta, C. M., Hack, H. R., Kuklis, C., Soman, S., King, J., Peachman, K. K., Kim, D., Chen, W. H., ... Pitt, M. L. M. (2022). A SARS-CoV-2 Spike Ferritin Nanoparticle Vaccine Is Protective and Promotes a Strong Immunological Response in the Cynomolgus Macaque Coronavirus Disease 2019 (COVID-19) Model. Vaccines, 10(5), Article 717. https://doi.org/10.3390/vaccines10050717

@article{343e2be14afd4154a2f48d55d122ea4a,

title = "A SARS-CoV-2 Spike Ferritin Nanoparticle Vaccine Is Protective and Promotes a Strong Immunological Response in the Cynomolgus Macaque Coronavirus Disease 2019 (COVID-19) Model",

abstract = "The COVID-19 pandemic has had a staggering impact on social, economic, and public health systems worldwide. Vaccine development and mobilization against SARS-CoV-2 (the etio-logic agent of COVID-19) has been rapid. However, novel strategies are still necessary to slow the pandemic, and this includes new approaches to vaccine development and/or delivery that will improve vaccination compliance and demonstrate efficacy against emerging variants. Here, we report on the immunogenicity and efficacy of a SARS-CoV-2 vaccine comprising stabilized, pre-fusion spike protein trimers displayed on a ferritin nanoparticle (SpFN) adjuvanted with either conven-tional aluminum hydroxide or the Army Liposomal Formulation QS-21 (ALFQ) in a cynomolgus macaque COVID-19 model. Vaccination resulted in robust cell-mediated and humoral responses and a significant reduction in lung lesions following SARS-CoV-2 infection. The strength of the immune response suggests that dose sparing through reduced or single dosing in primates may be possible with this vaccine. Overall, the data support further evaluation of SpFN as a SARS-CoV-2 protein-based vaccine candidate with attention to fractional dosing and schedule optimization.",

keywords = "Army Liposomal Formulation QS-21, COVID-19, SARS-CoV-2, SpFN, aluminum hy-droxide, ferritin nanoparticle, primate, vaccine",

author = "Johnston, {Sara C.} and Ricks, {Keersten M.} and Ines Lakhal-Naouar and Alexandra Jay and Caroline Subra and Raymond, {Jo Lynne} and King, {Hannah A.D.} and Franco Rossi and Clements, {Tamara L.} and David Fetterer and Samantha Tostenson and Cincotta, {Camila Macedo} and Hack, {Holly R.} and Caitlin Kuklis and Sandrine Soman and Jocelyn King and Peachman, {Kristina K.} and Dohoon Kim and Chen, {Wei Hung} and Sankhala, {Rajeshwer S.} and Martinez, {Elizabeth J.} and Agnes Hajduczki and Chang, {William C.} and Misook Choe and Thomas, {Paul V.} and Peterson, {Caroline E.} and Alexander Anderson and Isabella Swafford and Currier, {Jeffrey R.} and Dominic Paquin-Proulx and Jagodzinski, {Linda L.} and Matyas, {Gary R.} and Mangala Rao and Gromowski, {Gregory D.} and Peel, {Sheila A.} and Lauren White and Smith, {Jeffrey M.} and Hooper, {Jay W.} and Michael, {Nelson L.} and Kayvon Modjarrad and Joyce, {M. Gordon} and Aysegul Nalca and Bolton, {Diane L.} and Pitt, {Margaret L.M.}",

year = "2022",

month = may,

doi = "10.3390/vaccines10050717",

language = "English",

volume = "10",

journal = "Vaccines",

issn = "2076-393X",

number = "5",

}

Johnston, SC, Ricks, KM, Lakhal-Naouar, I, Jay, A, Subra, C, Raymond, JL, King, HAD, Rossi, F, Clements, TL, Fetterer, D, Tostenson, S, Cincotta, CM, Hack, HR, Kuklis, C, Soman, S, King, J, Peachman, KK, Kim, D, Chen, WH, Sankhala, RS, Martinez, EJ, Hajduczki, A, Chang, WC, Choe, M, Thomas, PV, Peterson, CE, Anderson, A, Swafford, I, Currier, JR, Paquin-Proulx, D, Jagodzinski, LL, Matyas, GR, Rao, M, Gromowski, GD, Peel, SA, White, L, Smith, JM, Hooper, JW, Michael, NL, Modjarrad, K, Joyce, MG, Nalca, A, Bolton, DL & Pitt, MLM 2022, 'A SARS-CoV-2 Spike Ferritin Nanoparticle Vaccine Is Protective and Promotes a Strong Immunological Response in the Cynomolgus Macaque Coronavirus Disease 2019 (COVID-19) Model', Vaccines, vol. 10, no. 5, 717. https://doi.org/10.3390/vaccines10050717

TY - JOUR

T1 - A SARS-CoV-2 Spike Ferritin Nanoparticle Vaccine Is Protective and Promotes a Strong Immunological Response in the Cynomolgus Macaque Coronavirus Disease 2019 (COVID-19) Model

AU - Johnston, Sara C.

AU - Ricks, Keersten M.

AU - Lakhal-Naouar, Ines

AU - Jay, Alexandra

AU - Subra, Caroline

AU - Raymond, Jo Lynne

AU - King, Hannah A.D.

AU - Rossi, Franco

AU - Clements, Tamara L.

AU - Fetterer, David

AU - Tostenson, Samantha

AU - Cincotta, Camila Macedo

AU - Hack, Holly R.

AU - Kuklis, Caitlin

AU - Soman, Sandrine

AU - King, Jocelyn

AU - Peachman, Kristina K.

AU - Kim, Dohoon

AU - Chen, Wei Hung

AU - Sankhala, Rajeshwer S.

AU - Martinez, Elizabeth J.

AU - Hajduczki, Agnes

AU - Chang, William C.

AU - Choe, Misook

AU - Thomas, Paul V.

AU - Peterson, Caroline E.

AU - Anderson, Alexander

AU - Swafford, Isabella

AU - Currier, Jeffrey R.

AU - Paquin-Proulx, Dominic

AU - Jagodzinski, Linda L.

AU - Matyas, Gary R.

AU - Rao, Mangala

AU - Gromowski, Gregory D.

AU - Peel, Sheila A.

AU - White, Lauren

AU - Smith, Jeffrey M.

AU - Hooper, Jay W.

AU - Michael, Nelson L.

AU - Modjarrad, Kayvon

AU - Joyce, M. Gordon

AU - Nalca, Aysegul

AU - Bolton, Diane L.

AU - Pitt, Margaret L.M.

PY - 2022/5

Y1 - 2022/5

N2 - The COVID-19 pandemic has had a staggering impact on social, economic, and public health systems worldwide. Vaccine development and mobilization against SARS-CoV-2 (the etio-logic agent of COVID-19) has been rapid. However, novel strategies are still necessary to slow the pandemic, and this includes new approaches to vaccine development and/or delivery that will improve vaccination compliance and demonstrate efficacy against emerging variants. Here, we report on the immunogenicity and efficacy of a SARS-CoV-2 vaccine comprising stabilized, pre-fusion spike protein trimers displayed on a ferritin nanoparticle (SpFN) adjuvanted with either conven-tional aluminum hydroxide or the Army Liposomal Formulation QS-21 (ALFQ) in a cynomolgus macaque COVID-19 model. Vaccination resulted in robust cell-mediated and humoral responses and a significant reduction in lung lesions following SARS-CoV-2 infection. The strength of the immune response suggests that dose sparing through reduced or single dosing in primates may be possible with this vaccine. Overall, the data support further evaluation of SpFN as a SARS-CoV-2 protein-based vaccine candidate with attention to fractional dosing and schedule optimization.

AB - The COVID-19 pandemic has had a staggering impact on social, economic, and public health systems worldwide. Vaccine development and mobilization against SARS-CoV-2 (the etio-logic agent of COVID-19) has been rapid. However, novel strategies are still necessary to slow the pandemic, and this includes new approaches to vaccine development and/or delivery that will improve vaccination compliance and demonstrate efficacy against emerging variants. Here, we report on the immunogenicity and efficacy of a SARS-CoV-2 vaccine comprising stabilized, pre-fusion spike protein trimers displayed on a ferritin nanoparticle (SpFN) adjuvanted with either conven-tional aluminum hydroxide or the Army Liposomal Formulation QS-21 (ALFQ) in a cynomolgus macaque COVID-19 model. Vaccination resulted in robust cell-mediated and humoral responses and a significant reduction in lung lesions following SARS-CoV-2 infection. The strength of the immune response suggests that dose sparing through reduced or single dosing in primates may be possible with this vaccine. Overall, the data support further evaluation of SpFN as a SARS-CoV-2 protein-based vaccine candidate with attention to fractional dosing and schedule optimization.

KW - Army Liposomal Formulation QS-21

KW - COVID-19

KW - SARS-CoV-2

KW - SpFN

KW - aluminum hy-droxide

KW - ferritin nanoparticle

KW - primate

KW - vaccine

UR - http://www.scopus.com/inward/record.url?scp=85130118465&partnerID=8YFLogxK

U2 - 10.3390/vaccines10050717

DO - 10.3390/vaccines10050717

M3 - Article

AN - SCOPUS:85130118465

SN - 2076-393X

VL - 10

JO - Vaccines

JF - Vaccines

IS - 5

M1 - 717

ER -

A SARS-CoV-2 Spike Ferritin Nanoparticle Vaccine Is Protective and Promotes a Strong Immunological Response in the Cynomolgus Macaque Coronavirus Disease 2019 (COVID-19) Model

Abstract

Keywords

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