A Simple and Rapid Method for the Purification of Ovine Pineal Arylalkylamine N‐Acetyltransferase

M. A.A. Namboodiri; M. J. Brownstein; Pierre Voisin; Joan L. Weller; David C. Klein

doi:10.1111/j.1471-4159.1987.tb04132.x

A Simple and Rapid Method for the Purification of Ovine Pineal Arylalkylamine N‐Acetyltransferase

M. A.A. Namboodiri, M. J. Brownstein, Pierre Voisin, Joan L. Weller, David C. Klein^*

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Abstract: A two‐step chromatographic procedure has been developed for the purification of ovine pineal arylalkylamine N‐acetyltransferase (EC 2.3.1.87), based on the principles of disulfide exchange and anion exchange. The enzyme from 20 ovine pineal glands can be purified about 500‐fold in a day; recovery is about 5%. Polyacrylamide gel electrophoretic analysis of the final preparation shows four major bands; one appears to be arylalkylamine N‐acetyl‐transferase.

Original language	English
Pages (from-to)	580-585
Number of pages	6
Journal	Journal of Neurochemistry
Volume	48
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-4159.1987.tb04132.x
State	Published - Feb 1987

Keywords

Anion exchange
Cystamine
Disulfide exchange
Fast protein liquid chromatography

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10.1111/j.1471-4159.1987.tb04132.x

Cite this

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title = "A Simple and Rapid Method for the Purification of Ovine Pineal Arylalkylamine N‐Acetyltransferase",

abstract = "Abstract: A two‐step chromatographic procedure has been developed for the purification of ovine pineal arylalkylamine N‐acetyltransferase (EC 2.3.1.87), based on the principles of disulfide exchange and anion exchange. The enzyme from 20 ovine pineal glands can be purified about 500‐fold in a day; recovery is about 5%. Polyacrylamide gel electrophoretic analysis of the final preparation shows four major bands; one appears to be arylalkylamine N‐acetyl‐transferase.",

keywords = "Anion exchange, Cystamine, Disulfide exchange, Fast protein liquid chromatography",

author = "Namboodiri, {M. A.A.} and Brownstein, {M. J.} and Pierre Voisin and Weller, {Joan L.} and Klein, {David C.}",

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T1 - A Simple and Rapid Method for the Purification of Ovine Pineal Arylalkylamine N‐Acetyltransferase

AU - Namboodiri, M. A.A.

AU - Brownstein, M. J.

AU - Voisin, Pierre

AU - Weller, Joan L.

AU - Klein, David C.

PY - 1987/2

Y1 - 1987/2

N2 - Abstract: A two‐step chromatographic procedure has been developed for the purification of ovine pineal arylalkylamine N‐acetyltransferase (EC 2.3.1.87), based on the principles of disulfide exchange and anion exchange. The enzyme from 20 ovine pineal glands can be purified about 500‐fold in a day; recovery is about 5%. Polyacrylamide gel electrophoretic analysis of the final preparation shows four major bands; one appears to be arylalkylamine N‐acetyl‐transferase.

AB - Abstract: A two‐step chromatographic procedure has been developed for the purification of ovine pineal arylalkylamine N‐acetyltransferase (EC 2.3.1.87), based on the principles of disulfide exchange and anion exchange. The enzyme from 20 ovine pineal glands can be purified about 500‐fold in a day; recovery is about 5%. Polyacrylamide gel electrophoretic analysis of the final preparation shows four major bands; one appears to be arylalkylamine N‐acetyl‐transferase.

KW - Anion exchange

KW - Cystamine

KW - Disulfide exchange

KW - Fast protein liquid chromatography

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U2 - 10.1111/j.1471-4159.1987.tb04132.x

DO - 10.1111/j.1471-4159.1987.tb04132.x

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