A Sulfilimine Bond Identified in Collagen IV

Roberto Vanacore*, Amy Joan L. Ham, Markus Voehler, Charles R. Sanders, Thomas P. Conrads, Timothy D. Veenstra, Barry Sharpless, Philip E. Dawson, Billy G. Hudson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

190 Scopus citations

Abstract

Collagen IV networks are ancient proteins of basement membranes that underlie epithelia in metazoa from sponge to human. The networks provide structural integrity to tissues and serve as ligands for integrin cell-surface receptors. They are assembled by oligomerization of triple-helical protomers and are covalently crosslinked, a key reinforcement that stabilizes networks. We used Fourier-transform ion cyclotron resonance mass spectrometry and nuclear magnetic resonance spectroscopy to show that a sulfilimine bond (-S=N-) crosslinks hydroxylysine-211 and methionine93 of adjoining protomers, a bond not previously found in biomolecules. This bond, the nitrogen analog of a sulfoxide, appears to have arisen at the divergence of sponge and cnidaria, an adaptation of the extracellular matrix in response to mechanical stress in metazoan evolution.

Original languageEnglish
Pages (from-to)1230-1234
Number of pages5
JournalScience
Volume325
Issue number5945
DOIs
StatePublished - 2009
Externally publishedYes

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