Activation of pineal and brain acetyl-CoA hydrolase by cystamine: An apparent case of disulfide exchange

M. A.A. Namboodiri; J. T. Favilla; D. C. Klein

doi:10.1016/0006-291X(79)92002-3

Activation of pineal and brain acetyl-CoA hydrolase by cystamine: An apparent case of disulfide exchange

M. A.A. Namboodiri^*
, J. T. Favilla
, D. C. Klein

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Rat pineal acetyl-CoA hydrolase was activated about 5-fold by cystamine treatment (30 mM) at pH 6.8 and 10-fold at pH 8.5. Six other disulfides were found to be ineffective or to produce a small activation. Cystamine activation was not reversed when free cystamine was removed, but was reversed by treatment with DTT. Analysis of other tissues indicated acetyl-CoA hydrolase from rat brain, sheep pineal gland and chicken pineal gland could also be activated by cystamine. In contrast, cystamine activation of rat liver acetyl-CoA hydrolase was not seen.

Original language	English
Pages (from-to)	1166-1173
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	91
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(79)92002-3
State	Published - 14 Dec 1979

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title = "Activation of pineal and brain acetyl-CoA hydrolase by cystamine: An apparent case of disulfide exchange",

abstract = "Rat pineal acetyl-CoA hydrolase was activated about 5-fold by cystamine treatment (30 mM) at pH 6.8 and 10-fold at pH 8.5. Six other disulfides were found to be ineffective or to produce a small activation. Cystamine activation was not reversed when free cystamine was removed, but was reversed by treatment with DTT. Analysis of other tissues indicated acetyl-CoA hydrolase from rat brain, sheep pineal gland and chicken pineal gland could also be activated by cystamine. In contrast, cystamine activation of rat liver acetyl-CoA hydrolase was not seen.",

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T1 - Activation of pineal and brain acetyl-CoA hydrolase by cystamine

T2 - An apparent case of disulfide exchange

AU - Namboodiri, M. A.A.

AU - Favilla, J. T.

AU - Klein, D. C.

PY - 1979/12/14

Y1 - 1979/12/14

N2 - Rat pineal acetyl-CoA hydrolase was activated about 5-fold by cystamine treatment (30 mM) at pH 6.8 and 10-fold at pH 8.5. Six other disulfides were found to be ineffective or to produce a small activation. Cystamine activation was not reversed when free cystamine was removed, but was reversed by treatment with DTT. Analysis of other tissues indicated acetyl-CoA hydrolase from rat brain, sheep pineal gland and chicken pineal gland could also be activated by cystamine. In contrast, cystamine activation of rat liver acetyl-CoA hydrolase was not seen.

AB - Rat pineal acetyl-CoA hydrolase was activated about 5-fold by cystamine treatment (30 mM) at pH 6.8 and 10-fold at pH 8.5. Six other disulfides were found to be ineffective or to produce a small activation. Cystamine activation was not reversed when free cystamine was removed, but was reversed by treatment with DTT. Analysis of other tissues indicated acetyl-CoA hydrolase from rat brain, sheep pineal gland and chicken pineal gland could also be activated by cystamine. In contrast, cystamine activation of rat liver acetyl-CoA hydrolase was not seen.

UR - https://www.scopus.com/pages/publications/0018593157

U2 - 10.1016/0006-291X(79)92002-3

DO - 10.1016/0006-291X(79)92002-3

M3 - Article

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AN - SCOPUS:0018593157

SN - 0006-291X

VL - 91

SP - 1166

EP - 1173

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Activation of pineal and brain acetyl-CoA hydrolase by cystamine: An apparent case of disulfide exchange

Abstract

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