ADAR1 Zα domain P195A mutation activates the MDA5-dependent RNA-sensing signaling pathway in brain without decreasing overall RNA editing

Xinfeng Guo, Silvia Liu, Yi Sheng, Mazen Zenati, Timothy Billiar, Alan Herbert, Qingde Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Variants of the RNA-editing enzyme ADAR1 cause Aicardi-Goutières syndrome (AGS), in which severe inflammation occurs in the brain due to innate immune activation. Here, we analyze the RNA-editing status and innate immune activation in an AGS mouse model that carries the Adar P195A mutation in the N terminus of the ADAR1 p150 isoform, the equivalent of the P193A human Zα variant causal for disease. This mutation alone can cause interferon-stimulated gene (ISG) expression in the brain, especially in the periventricular areas, reflecting the pathologic feature of AGS. However, in these mice, ISG expression does not correlate with an overall decrease in RNA editing. Rather, the enhanced ISG expression in the brain due to the P195A mutant is dose dependent. Our findings indicate that ADAR1 can regulate innate immune responses through Z-RNA binding without changing overall RNA editing.

Original languageEnglish
Article number112733
JournalCell Reports
Volume42
Issue number7
DOIs
StatePublished - 25 Jul 2023
Externally publishedYes

Keywords

  • A-to-I RNA editing
  • ADAR1
  • AGS
  • Aicardi-Goutières syndrome
  • CP: Immunology
  • IFN-stimulated gene
  • ISG
  • MDA-5
  • RNA-sensing signaling pathway
  • Z-RNA
  • adenosine deaminase acting on RNA 1
  • melanoma differentiation-associated protein 5
  • neuroinflammation

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