TY - JOUR
T1 - Advances in proximal fluid proteomics for disease biomarker discovery
AU - Teng, Pang Ning
AU - Bateman, Nicholas W.
AU - Hood, Brian L.
AU - Conrads, Thomas P.
PY - 2010/12/3
Y1 - 2010/12/3
N2 - Although serum/plasma has been the preferred source for identification of disease biomarkers, these efforts have been met with little success, in large part due the relatively small number of highly abundant proteins that render the reliable detection of low abundant disease-related proteins challenging due to the expansive dynamic range of concentration of proteins in this sample. Proximal fluid, the fluid derived from the extracellular milieu of tissues, contains a large repertoire of shed and secreted proteins that are likely to be present at higher concentrations relative to plasma/serum. It is hypothesized that many, if not all, proximal fluid proteins exchange with peripheral circulation, which has provided significant motivation for utilizing proximal fluids as a primary sample source for protein biomarker discovery. The present review highlights recent advances in proximal fluid proteomics, including the various protocols utilized to harvest proximal fluids along with detailing the results from mass spectrometry- and antibody-based analyses.
AB - Although serum/plasma has been the preferred source for identification of disease biomarkers, these efforts have been met with little success, in large part due the relatively small number of highly abundant proteins that render the reliable detection of low abundant disease-related proteins challenging due to the expansive dynamic range of concentration of proteins in this sample. Proximal fluid, the fluid derived from the extracellular milieu of tissues, contains a large repertoire of shed and secreted proteins that are likely to be present at higher concentrations relative to plasma/serum. It is hypothesized that many, if not all, proximal fluid proteins exchange with peripheral circulation, which has provided significant motivation for utilizing proximal fluids as a primary sample source for protein biomarker discovery. The present review highlights recent advances in proximal fluid proteomics, including the various protocols utilized to harvest proximal fluids along with detailing the results from mass spectrometry- and antibody-based analyses.
UR - http://www.scopus.com/inward/record.url?scp=78649858218&partnerID=8YFLogxK
U2 - 10.1021/pr100904q
DO - 10.1021/pr100904q
M3 - Review article
C2 - 21028795
AN - SCOPUS:78649858218
SN - 1535-3893
VL - 9
SP - 6091
EP - 6100
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 12
ER -