TY - JOUR
T1 - Aging and reactivation of latent murine cytomegalovirus
AU - Schmader, Kenneth E.
AU - Rahija, Richard
AU - Porter, Kevin R.
AU - Daley, Gerald
AU - Hamilton, John D.
N1 - Funding Information:
Received II May 1992; revised 13 luly 1992. Grant support: Brookdale Foundation (K.E.S. is National Fellow); National Institutes of Health (RR-05405; AG-00526 to K.E.S.); Medical Research Service, Department of Veterans Affairs. Reprints or correspondence: Dr. Kenneth Schmader. Center for the Study of Aging and Human Development, Box 3469, Duke University Medical Center, Durham, NC 22710. • Present affiliation: Naval Medical Research Institute. Rockville. MD.
PY - 1992/12
Y1 - 1992/12
N2 - BALB/c mice were experimentally infected with murine cytomegalovirus (MCMV) to discover whether latent MCMV persisted in aging mice and to examine the effect of aging on MCMV reactivation. Latently infected mice received saline, cyclophosphamide, or allogeneic blood at 6 and 18 months of age. MCMV DNA was detected by polymerase chain reaction in submaxillary salivary gland biopsy specimens from saline-treated young and old mice. Evidence of MCMV reactivation was sought by culture of biopsy specimens and by MCMV IgG ELISA of pre- and posttreatment sera from all animals. Very few cyclophosphamide- or saline-treated mice reactivated MCMV at either age, but young transfused mice reactivated MCMV significantly more often than did old transfused mice. These experiments indicate that MCMV DNA persists in the salivary gland of aging mice but that the likelihood of MCMV reactivation does not increase with age.
AB - BALB/c mice were experimentally infected with murine cytomegalovirus (MCMV) to discover whether latent MCMV persisted in aging mice and to examine the effect of aging on MCMV reactivation. Latently infected mice received saline, cyclophosphamide, or allogeneic blood at 6 and 18 months of age. MCMV DNA was detected by polymerase chain reaction in submaxillary salivary gland biopsy specimens from saline-treated young and old mice. Evidence of MCMV reactivation was sought by culture of biopsy specimens and by MCMV IgG ELISA of pre- and posttreatment sera from all animals. Very few cyclophosphamide- or saline-treated mice reactivated MCMV at either age, but young transfused mice reactivated MCMV significantly more often than did old transfused mice. These experiments indicate that MCMV DNA persists in the salivary gland of aging mice but that the likelihood of MCMV reactivation does not increase with age.
UR - http://www.scopus.com/inward/record.url?scp=0026494760&partnerID=8YFLogxK
U2 - 10.1093/infdis/166.6.1403
DO - 10.1093/infdis/166.6.1403
M3 - Article
C2 - 1331252
AN - SCOPUS:0026494760
SN - 0022-1899
VL - 166
SP - 1403
EP - 1407
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -