All amacrine neurons of the rat retina show diurnal and circadian rhythms of parvalbumin immunoreactivity

Robert Gábriel; Joseph Lesauter; Tamás Bánvölgyi; György Petrovics; Rae Silver; Paul Witkovsky

doi:10.1007/s00441-003-0785-2

All amacrine neurons of the rat retina show diurnal and circadian rhythms of parvalbumin immunoreactivity

Robert Gábriel^*, Joseph Lesauter, Tamás Bánvölgyi, György Petrovics, Rae Silver, Paul Witkovsky

^*Corresponding author for this work

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Abstract

We investigated parvalbumin immunoreactivity (PA-IR) in the retinas of rats maintained on a 12:12 h light:dark cycle, or after being placed in constant darkness for 24-72 h. Retinas were harvested at zeitgeber and circadian times 02:00, 06:00, 10:00, 14:00, 18:00 and 22:00 h. PA-IR was found primarily in retinal amacrine cells of the AII subtype. In a light/dark cycle, PA-IR showed a clear rhythm, with a low near zeitgeber time (ZT) 10:00 h and a peak near ZT 18:00 h. The ratio of immunofluorescence intensities at these timepoints was >15-fold. When animals were kept in complete darkness for 1-3 days, the rhythm of PA-IR was still preserved, but was progressively reduced in amplitude. The rhythm of PA-IR inferred from immunohistochemical data was confirmed by Western blots. We conclude that PA-IR in the rat retina shows an underlying circadian rhythm that is enhanced by cyclic light. The regulation may involve translocation of the protein between cell compartments and/or new protein synthesis.

Original language	English
Pages (from-to)	181-186
Number of pages	6
Journal	Cell and Tissue Research
Volume	315
Issue number	2
DOIs	https://doi.org/10.1007/s00441-003-0785-2
State	Published - Feb 2004

Keywords

Calcium-binding proteins
Immunocytochemistry
Inner plexiform layer
Rat
Western blots

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title = "All amacrine neurons of the rat retina show diurnal and circadian rhythms of parvalbumin immunoreactivity",

abstract = "We investigated parvalbumin immunoreactivity (PA-IR) in the retinas of rats maintained on a 12:12 h light:dark cycle, or after being placed in constant darkness for 24-72 h. Retinas were harvested at zeitgeber and circadian times 02:00, 06:00, 10:00, 14:00, 18:00 and 22:00 h. PA-IR was found primarily in retinal amacrine cells of the AII subtype. In a light/dark cycle, PA-IR showed a clear rhythm, with a low near zeitgeber time (ZT) 10:00 h and a peak near ZT 18:00 h. The ratio of immunofluorescence intensities at these timepoints was >15-fold. When animals were kept in complete darkness for 1-3 days, the rhythm of PA-IR was still preserved, but was progressively reduced in amplitude. The rhythm of PA-IR inferred from immunohistochemical data was confirmed by Western blots. We conclude that PA-IR in the rat retina shows an underlying circadian rhythm that is enhanced by cyclic light. The regulation may involve translocation of the protein between cell compartments and/or new protein synthesis.",

keywords = "Calcium-binding proteins, Immunocytochemistry, Inner plexiform layer, Rat, Western blots",

author = "Robert G{\'a}briel and Joseph Lesauter and Tam{\'a}s B{\'a}nv{\"o}lgyi and Gy{\"o}rgy Petrovics and Rae Silver and Paul Witkovsky",

note = "Funding Information: This study was supported by an OTKA grant (T 34160), NIH grants NS 37919 (R.S.) and ET 03570, NSF grant IBN-96418886 (R.S.), and grants from the Helen Hoffritz Charitable Trust and Research to Prevent Blindness, Inc. R.G. was also in receipt of a J nos Bolyai fellowship",

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T1 - All amacrine neurons of the rat retina show diurnal and circadian rhythms of parvalbumin immunoreactivity

AU - Gábriel, Robert

AU - Lesauter, Joseph

AU - Bánvölgyi, Tamás

AU - Petrovics, György

AU - Silver, Rae

AU - Witkovsky, Paul

N1 - Funding Information: This study was supported by an OTKA grant (T 34160), NIH grants NS 37919 (R.S.) and ET 03570, NSF grant IBN-96418886 (R.S.), and grants from the Helen Hoffritz Charitable Trust and Research to Prevent Blindness, Inc. R.G. was also in receipt of a J nos Bolyai fellowship

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Y1 - 2004/2

N2 - We investigated parvalbumin immunoreactivity (PA-IR) in the retinas of rats maintained on a 12:12 h light:dark cycle, or after being placed in constant darkness for 24-72 h. Retinas were harvested at zeitgeber and circadian times 02:00, 06:00, 10:00, 14:00, 18:00 and 22:00 h. PA-IR was found primarily in retinal amacrine cells of the AII subtype. In a light/dark cycle, PA-IR showed a clear rhythm, with a low near zeitgeber time (ZT) 10:00 h and a peak near ZT 18:00 h. The ratio of immunofluorescence intensities at these timepoints was >15-fold. When animals were kept in complete darkness for 1-3 days, the rhythm of PA-IR was still preserved, but was progressively reduced in amplitude. The rhythm of PA-IR inferred from immunohistochemical data was confirmed by Western blots. We conclude that PA-IR in the rat retina shows an underlying circadian rhythm that is enhanced by cyclic light. The regulation may involve translocation of the protein between cell compartments and/or new protein synthesis.

AB - We investigated parvalbumin immunoreactivity (PA-IR) in the retinas of rats maintained on a 12:12 h light:dark cycle, or after being placed in constant darkness for 24-72 h. Retinas were harvested at zeitgeber and circadian times 02:00, 06:00, 10:00, 14:00, 18:00 and 22:00 h. PA-IR was found primarily in retinal amacrine cells of the AII subtype. In a light/dark cycle, PA-IR showed a clear rhythm, with a low near zeitgeber time (ZT) 10:00 h and a peak near ZT 18:00 h. The ratio of immunofluorescence intensities at these timepoints was >15-fold. When animals were kept in complete darkness for 1-3 days, the rhythm of PA-IR was still preserved, but was progressively reduced in amplitude. The rhythm of PA-IR inferred from immunohistochemical data was confirmed by Western blots. We conclude that PA-IR in the rat retina shows an underlying circadian rhythm that is enhanced by cyclic light. The regulation may involve translocation of the protein between cell compartments and/or new protein synthesis.

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All amacrine neurons of the rat retina show diurnal and circadian rhythms of parvalbumin immunoreactivity

Abstract

Keywords

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