TY - JOUR
T1 - Amylase and lipase detection in hemorrhaged animals treated with HBOC-201
AU - Arnaud, Françoise
AU - McCarron, Richard
AU - Freilich, Daniel
N1 - Funding Information:
1Biopure Corp is now OPK and it is located in Cambridge. MA. This work was performed at Naval Medical Research Center, Silver Spring, MD. The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the U.S. Government. U.S. Government employees as part of their official duties did this work; Copyright protection under Title 17 U.S.C &105 is not available for any work of the United Stated Government. This work was funded from ONR Work Unit No. 602236N.4426.W26.A0241. HBOC-201 and Hextend were provided by Biopure Corp., Cambridge, MA, and Abbott Laboratories, Chicago, IL, respectively. We are thankful to Yao Yang and Mike Hammett for technical assistance. Address correspondence to Françoise Arnaud, PhD., 503 Robert Grant Avenue, Operational and Undersea Medicine, Naval Medical Research Center, Silver Spring, MD 20910-7500, USA. E-mail: [email protected]
PY - 2011/6
Y1 - 2011/6
N2 - HBOC-201 may alter lipase and amylase detection on chemistry analyzers using optical methods and affect pancreatic function after trauma. Amylase and lipase measurements were correlated against HBOC-201 to evaluate interference on samples spiked with 0-6g/dL HBOC-201. The detection threshold was 2.5g/dL or none when measured, respectively, on Vitros 250 or Advia 1650 instruments. Amylase and lipase from blood samples collected from 55% EBV hemorrhaged Yucatan min-pigs showed peaks around 24-48 hours. Amylase increase was not significant between treatments but lipase was higher in HBOC-201-treated animals. Animals particularly affected by the injury had elevated enzymes after hemorrhagic shock, without significant clinical consequences.
AB - HBOC-201 may alter lipase and amylase detection on chemistry analyzers using optical methods and affect pancreatic function after trauma. Amylase and lipase measurements were correlated against HBOC-201 to evaluate interference on samples spiked with 0-6g/dL HBOC-201. The detection threshold was 2.5g/dL or none when measured, respectively, on Vitros 250 or Advia 1650 instruments. Amylase and lipase from blood samples collected from 55% EBV hemorrhaged Yucatan min-pigs showed peaks around 24-48 hours. Amylase increase was not significant between treatments but lipase was higher in HBOC-201-treated animals. Animals particularly affected by the injury had elevated enzymes after hemorrhagic shock, without significant clinical consequences.
KW - HBOC
KW - clinical chemistry
KW - hemorrhage
KW - interference
KW - pancreas
KW - trauma
UR - http://www.scopus.com/inward/record.url?scp=79956159032&partnerID=8YFLogxK
U2 - 10.3109/10731199.2010.516260
DO - 10.3109/10731199.2010.516260
M3 - Article
C2 - 21171938
AN - SCOPUS:79956159032
SN - 1073-1199
VL - 39
SP - 155
EP - 161
JO - Artificial Cells, Blood Substitutes, and Biotechnology
JF - Artificial Cells, Blood Substitutes, and Biotechnology
IS - 3
ER -