An immunocytometric assay based on dengue infection via DC-SIGN permits rapid measurement of anti-dengue neutralizing antibodies

Nicole C. Martin, Jorge Pardo, Monika Simmons, Jeffrey A. Tjaden, Susana Widjaja, Mary A. Marovich, Wellington Sun, Kevin R. Porter, Timothy H. Burgess*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Dengue remains a global public health threat and development of a safe and effective vaccine is a principal public health goal. The primary correlate of immunity is thought to be neutralizing antibodies. Currently, the plaque reduction neutralization test (PRNT) is the gold standard measure of dengue neutralizing antibody responses, but this test is limited by time-consuming performance. In addition, some feel that use of viral strains adapted to grow in Vero or BHK cells may not accurately reflect protective responses. A human cell line transfected to express a putative natural dengue receptor, DC-SIGN (CD209), was used to measure antibody-mediated dengue neutralization. Using neutralizing monoclonal antibodies, immune sera, and laboratory adapted dengue viruses, serotype-specific neutralizing activity was demonstrated similar to that seen in the Vero PRNT. Importantly, serotype-specific neutralizing activity against recently isolated dengue strains with less heterotypic cross-neutralization than laboratory adapted viruses was also demonstrated.

Original languageEnglish
Pages (from-to)74-85
Number of pages12
JournalJournal of Virological Methods
Volume134
Issue number1-2
DOIs
StatePublished - Jun 2006

Keywords

  • DC-SIGN
  • Dengue antibodies
  • Dengue virus
  • Immunocytofluorimetry
  • Neutralization
  • PRNT

Fingerprint

Dive into the research topics of 'An immunocytometric assay based on dengue infection via DC-SIGN permits rapid measurement of anti-dengue neutralizing antibodies'. Together they form a unique fingerprint.

Cite this