An Integrated High-Resolution Physical Map of the DPC/BRCA2 Region at Chromosome 13q12

Mieke Schutte, Ester Rozenblum, Christopher A. Moskaluk, A. T.M.Shamsul Hoque, Stephan A. Hahn, Luis T. da Costa, Scott E. Kern*, Scott E. Kern*, Xiaoping Guan, Pieter J. deJong

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

We identified a homozygous deletion in a pancreatic carcinoma (DPC) that localized to a 1-cM region at chromosome 13ql2.3, which lay within the 6-cM locus of familial breast cancer susceptibility (BRCA2). Here we present a physical map of the region, consisting of YAC, PAC, and cosmid contigs. The YAC contig comprises 16 clones that together span the entire BRCA2 region. The PAC contig comprises 22 clones that together span the DPC region. Seventy cosmid clones were localized within and near the DPC region. Thirty-five sequence-tagged sites were defined and localized within the map. The map indicates the size of the DPC region to be near 250 kb, and provides mapped and cloned resources for the search for the putative tumor suppressor gene(s) in the region.

Original languageEnglish
Pages (from-to)4570-4574
Number of pages5
JournalCancer Research
Volume55
Issue number20
StatePublished - 15 Oct 1995
Externally publishedYes

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