An Integrated High-Resolution Physical Map of the DPC/BRCA2 Region at Chromosome 13q12

Mieke Schutte; Ester Rozenblum; Christopher A. Moskaluk; A. T.M.Shamsul Hoque; Stephan A. Hahn; Luis T. da Costa; Scott E. Kern; Scott E. Kern; Xiaoping Guan; Pieter J. deJong

An Integrated High-Resolution Physical Map of the DPC/BRCA2 Region at Chromosome 13q12

Mieke Schutte, Ester Rozenblum, Christopher A. Moskaluk, A. T.M.Shamsul Hoque, Stephan A. Hahn, Luis T. da Costa, Scott E. Kern^*, Scott E. Kern^*, Xiaoping Guan, Pieter J. deJong

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

We identified a homozygous deletion in a pancreatic carcinoma (DPC) that localized to a 1-cM region at chromosome 13ql2.3, which lay within the 6-cM locus of familial breast cancer susceptibility (BRCA2). Here we present a physical map of the region, consisting of YAC, PAC, and cosmid contigs. The YAC contig comprises 16 clones that together span the entire BRCA2 region. The PAC contig comprises 22 clones that together span the DPC region. Seventy cosmid clones were localized within and near the DPC region. Thirty-five sequence-tagged sites were defined and localized within the map. The map indicates the size of the DPC region to be near 250 kb, and provides mapped and cloned resources for the search for the putative tumor suppressor gene(s) in the region.

Original language	English
Pages (from-to)	4570-4574
Number of pages	5
Journal	Cancer Research
Volume	55
Issue number	20
State	Published - 15 Oct 1995
Externally published	Yes

Cite this

@article{8499b41944144fb59d0452d86c659444,

title = "An Integrated High-Resolution Physical Map of the DPC/BRCA2 Region at Chromosome 13q12",

abstract = "We identified a homozygous deletion in a pancreatic carcinoma (DPC) that localized to a 1-cM region at chromosome 13ql2.3, which lay within the 6-cM locus of familial breast cancer susceptibility (BRCA2). Here we present a physical map of the region, consisting of YAC, PAC, and cosmid contigs. The YAC contig comprises 16 clones that together span the entire BRCA2 region. The PAC contig comprises 22 clones that together span the DPC region. Seventy cosmid clones were localized within and near the DPC region. Thirty-five sequence-tagged sites were defined and localized within the map. The map indicates the size of the DPC region to be near 250 kb, and provides mapped and cloned resources for the search for the putative tumor suppressor gene(s) in the region.",

author = "Mieke Schutte and Ester Rozenblum and Moskaluk, {Christopher A.} and Hoque, {A. T.M.Shamsul} and Hahn, {Stephan A.} and {da Costa}, {Luis T.} and Kern, {Scott E.} and Kern, {Scott E.} and Xiaoping Guan and deJong, {Pieter J.}",

year = "1995",

month = oct,

day = "15",

language = "English",

volume = "55",

pages = "4570--4574",

journal = "Cancer Research",

issn = "0008-5472",

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TY - JOUR

T1 - An Integrated High-Resolution Physical Map of the DPC/BRCA2 Region at Chromosome 13q12

AU - Schutte, Mieke

AU - Rozenblum, Ester

AU - Moskaluk, Christopher A.

AU - Hoque, A. T.M.Shamsul

AU - Hahn, Stephan A.

AU - da Costa, Luis T.

AU - Kern, Scott E.

AU - Guan, Xiaoping

AU - deJong, Pieter J.

PY - 1995/10/15

Y1 - 1995/10/15

N2 - We identified a homozygous deletion in a pancreatic carcinoma (DPC) that localized to a 1-cM region at chromosome 13ql2.3, which lay within the 6-cM locus of familial breast cancer susceptibility (BRCA2). Here we present a physical map of the region, consisting of YAC, PAC, and cosmid contigs. The YAC contig comprises 16 clones that together span the entire BRCA2 region. The PAC contig comprises 22 clones that together span the DPC region. Seventy cosmid clones were localized within and near the DPC region. Thirty-five sequence-tagged sites were defined and localized within the map. The map indicates the size of the DPC region to be near 250 kb, and provides mapped and cloned resources for the search for the putative tumor suppressor gene(s) in the region.

AB - We identified a homozygous deletion in a pancreatic carcinoma (DPC) that localized to a 1-cM region at chromosome 13ql2.3, which lay within the 6-cM locus of familial breast cancer susceptibility (BRCA2). Here we present a physical map of the region, consisting of YAC, PAC, and cosmid contigs. The YAC contig comprises 16 clones that together span the entire BRCA2 region. The PAC contig comprises 22 clones that together span the DPC region. Seventy cosmid clones were localized within and near the DPC region. Thirty-five sequence-tagged sites were defined and localized within the map. The map indicates the size of the DPC region to be near 250 kb, and provides mapped and cloned resources for the search for the putative tumor suppressor gene(s) in the region.

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M3 - Article

C2 - 7553631

AN - SCOPUS:0028864588

SN - 0008-5472

VL - 55

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EP - 4574

JO - Cancer Research

JF - Cancer Research

IS - 20

ER -

An Integrated High-Resolution Physical Map of the DPC/BRCA2 Region at Chromosome 13q12

Abstract

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