Analyzing gene function in adult long-term hematopoietic stem cells using the interferon inducible Mx1-Cre mouse system

Kristbjorn Orri Gudmundsson; Kevin Oakley; Yufen Han; Yang Du

doi:10.1007/978-1-4939-1215-5_17

Analyzing gene function in adult long-term hematopoietic stem cells using the interferon inducible Mx1-Cre mouse system

Kristbjorn Orri Gudmundsson^*, Kevin Oakley, Yufen Han, Yang Du

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

Long-term hematopoietic stem cells (LT-HSCs) have the ability to self-renew and differentiate into all blood cell lineages. Understanding the genetic networks that regulate LT-HSC function in the adult bone marrow requires inducible gene targeting and bone marrow transplantations. In this chapter we describe the use of the inducible Mx1-Cre mouse model to delete genes in LT-HSCs and methodologies for examining the function of LT-HSCs following deletion.

Original language	English
Title of host publication	Mouse Genetics
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	313-325
Number of pages	13
ISBN (Print)	9781493912148
DOIs	https://doi.org/10.1007/978-1-4939-1215-5_17
State	Published - 2014

Publication series

Name	Methods in Molecular Biology
Volume	1194
ISSN (Print)	1064-3745

Keywords

Conditional gene deletion
Inducible Cre
Long-term hematopoietic stem cells
Mx1-Cre
Self-renewal
Transplantation

Access to Document

10.1007/978-1-4939-1215-5_17

Cite this

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abstract = "Long-term hematopoietic stem cells (LT-HSCs) have the ability to self-renew and differentiate into all blood cell lineages. Understanding the genetic networks that regulate LT-HSC function in the adult bone marrow requires inducible gene targeting and bone marrow transplantations. In this chapter we describe the use of the inducible Mx1-Cre mouse model to delete genes in LT-HSCs and methodologies for examining the function of LT-HSCs following deletion.",

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Analyzing gene function in adult long-term hematopoietic stem cells using the interferon inducible Mx1-Cre mouse system. / Gudmundsson, Kristbjorn Orri; Oakley, Kevin; Han, Yufen et al.
Mouse Genetics: Methods and Protocols. Humana Press Inc., 2014. p. 313-325 (Methods in Molecular Biology; Vol. 1194).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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