Mobilization of intracellular calcium from inositol-1,4,5-triphosphate (IP3)-sensitive endoplasmic reticulum (ER) stores plays a prominent role in brain function. Mice heterozygous for the annexin A7 (Anx7) gene have a profound reduction in IP3 receptor function in pancreatic islets along with defective insulin secretion. We examined IP3-sensitive calcium pools in the brains of Anx7 (+/-) mice by utilizing ATP/Mg 2+-dependent 45Ca 2+ uptake into brain membrane preparations and tissue sections. Although the Anx7 (+/-) mouse brain displayed similar levels of IP3 binding sites and thapsigargin-sensitive 45Ca 2+ uptake as that seen in wild-type mouse brain, the Anx7 (+/-) mouse brain Ca 2+ pools showed markedly reduced sensitivity to IP3. A potent and saturable Ca 2+-releasing effect of recombinant ANX7 protein was demonstrated in mouse and rat brain membrane preparations, which was additive with that of IP3. We propose that ANX7 mobilizes Ca 2+ from an endoplasmic reticulum-like pool, which can be recruited to enhance IP3-mediated Ca 2+ release.
|Number of pages||10|
|Journal||Biochimica et Biophysica Acta - Molecular Cell Research|
|State||Published - 6 Dec 2004|
- Annexin 7
- Endoplasmic reticulum
- IP3 receptor
- Knockout mouse