Annexin 7 mobilizes calcium from endoplasmic reticulum stores in brain

W. D. Watson; M. Srivastava; X. Leighton; M. Glasman; M. Faraday; L. H. Fossam; H. B. Pollard; A. Verma

doi:10.1016/j.bbamcr.2004.10.008

Annexin 7 mobilizes calcium from endoplasmic reticulum stores in brain

W. D. Watson, M. Srivastava, X. Leighton, M. Glasman, M. Faraday, L. H. Fossam, H. B. Pollard, A. Verma

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Mobilization of intracellular calcium from inositol-1,4,5-triphosphate (IP3)-sensitive endoplasmic reticulum (ER) stores plays a prominent role in brain function. Mice heterozygous for the annexin A7 (Anx7) gene have a profound reduction in IP3 receptor function in pancreatic islets along with defective insulin secretion. We examined IP3-sensitive calcium pools in the brains of Anx7 (+/-) mice by utilizing ATP/Mg ²⁺-dependent ⁴⁵Ca ²⁺ uptake into brain membrane preparations and tissue sections. Although the Anx7 (+/-) mouse brain displayed similar levels of IP3 binding sites and thapsigargin-sensitive ⁴⁵Ca ²⁺ uptake as that seen in wild-type mouse brain, the Anx7 (+/-) mouse brain Ca ²⁺ pools showed markedly reduced sensitivity to IP3. A potent and saturable Ca ²⁺-releasing effect of recombinant ANX7 protein was demonstrated in mouse and rat brain membrane preparations, which was additive with that of IP3. We propose that ANX7 mobilizes Ca ²⁺ from an endoplasmic reticulum-like pool, which can be recruited to enhance IP3-mediated Ca ²⁺ release.

Original language	English
Pages (from-to)	151-160
Number of pages	10
Journal	Biochimica et Biophysica Acta - Molecular Cell Research
Volume	1742
Issue number	1-3
DOIs	https://doi.org/10.1016/j.bbamcr.2004.10.008
State	Published - 6 Dec 2004
Externally published	Yes

Keywords

Annexin 7
Brain
Calcium
Endoplasmic reticulum
IP3 receptor
Knockout mouse
Signaling

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10.1016/j.bbamcr.2004.10.008

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title = "Annexin 7 mobilizes calcium from endoplasmic reticulum stores in brain",

abstract = "Mobilization of intracellular calcium from inositol-1,4,5-triphosphate (IP3)-sensitive endoplasmic reticulum (ER) stores plays a prominent role in brain function. Mice heterozygous for the annexin A7 (Anx7) gene have a profound reduction in IP3 receptor function in pancreatic islets along with defective insulin secretion. We examined IP3-sensitive calcium pools in the brains of Anx7 (+/-) mice by utilizing ATP/Mg 2+-dependent 45Ca 2+ uptake into brain membrane preparations and tissue sections. Although the Anx7 (+/-) mouse brain displayed similar levels of IP3 binding sites and thapsigargin-sensitive 45Ca 2+ uptake as that seen in wild-type mouse brain, the Anx7 (+/-) mouse brain Ca 2+ pools showed markedly reduced sensitivity to IP3. A potent and saturable Ca 2+-releasing effect of recombinant ANX7 protein was demonstrated in mouse and rat brain membrane preparations, which was additive with that of IP3. We propose that ANX7 mobilizes Ca 2+ from an endoplasmic reticulum-like pool, which can be recruited to enhance IP3-mediated Ca 2+ release.",

keywords = "Annexin 7, Brain, Calcium, Endoplasmic reticulum, IP3 receptor, Knockout mouse, Signaling",

author = "Watson, {W. D.} and M. Srivastava and X. Leighton and M. Glasman and M. Faraday and Fossam, {L. H.} and Pollard, {H. B.} and A. Verma",

note = "Funding Information: This work was supported by DOD grants F292AH, F192AN, Department of Clinical Investigations WU no. 0371020, Walter Reed Army Medical Center, and USUHS grant C070IV.",

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AU - Watson, W. D.

AU - Srivastava, M.

AU - Leighton, X.

AU - Glasman, M.

AU - Faraday, M.

AU - Fossam, L. H.

AU - Pollard, H. B.

AU - Verma, A.

N1 - Funding Information: This work was supported by DOD grants F292AH, F192AN, Department of Clinical Investigations WU no. 0371020, Walter Reed Army Medical Center, and USUHS grant C070IV.

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N2 - Mobilization of intracellular calcium from inositol-1,4,5-triphosphate (IP3)-sensitive endoplasmic reticulum (ER) stores plays a prominent role in brain function. Mice heterozygous for the annexin A7 (Anx7) gene have a profound reduction in IP3 receptor function in pancreatic islets along with defective insulin secretion. We examined IP3-sensitive calcium pools in the brains of Anx7 (+/-) mice by utilizing ATP/Mg 2+-dependent 45Ca 2+ uptake into brain membrane preparations and tissue sections. Although the Anx7 (+/-) mouse brain displayed similar levels of IP3 binding sites and thapsigargin-sensitive 45Ca 2+ uptake as that seen in wild-type mouse brain, the Anx7 (+/-) mouse brain Ca 2+ pools showed markedly reduced sensitivity to IP3. A potent and saturable Ca 2+-releasing effect of recombinant ANX7 protein was demonstrated in mouse and rat brain membrane preparations, which was additive with that of IP3. We propose that ANX7 mobilizes Ca 2+ from an endoplasmic reticulum-like pool, which can be recruited to enhance IP3-mediated Ca 2+ release.

AB - Mobilization of intracellular calcium from inositol-1,4,5-triphosphate (IP3)-sensitive endoplasmic reticulum (ER) stores plays a prominent role in brain function. Mice heterozygous for the annexin A7 (Anx7) gene have a profound reduction in IP3 receptor function in pancreatic islets along with defective insulin secretion. We examined IP3-sensitive calcium pools in the brains of Anx7 (+/-) mice by utilizing ATP/Mg 2+-dependent 45Ca 2+ uptake into brain membrane preparations and tissue sections. Although the Anx7 (+/-) mouse brain displayed similar levels of IP3 binding sites and thapsigargin-sensitive 45Ca 2+ uptake as that seen in wild-type mouse brain, the Anx7 (+/-) mouse brain Ca 2+ pools showed markedly reduced sensitivity to IP3. A potent and saturable Ca 2+-releasing effect of recombinant ANX7 protein was demonstrated in mouse and rat brain membrane preparations, which was additive with that of IP3. We propose that ANX7 mobilizes Ca 2+ from an endoplasmic reticulum-like pool, which can be recruited to enhance IP3-mediated Ca 2+ release.

KW - Annexin 7

KW - Brain

KW - Calcium

KW - Endoplasmic reticulum

KW - IP3 receptor

KW - Knockout mouse

KW - Signaling

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Annexin 7 mobilizes calcium from endoplasmic reticulum stores in brain

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