Annexin 7 mobilizes calcium from endoplasmic reticulum stores in brain

W. D. Watson, M. Srivastava, X. Leighton, M. Glasman, M. Faraday, L. H. Fossam, H. B. Pollard, A. Verma

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Mobilization of intracellular calcium from inositol-1,4,5-triphosphate (IP3)-sensitive endoplasmic reticulum (ER) stores plays a prominent role in brain function. Mice heterozygous for the annexin A7 (Anx7) gene have a profound reduction in IP3 receptor function in pancreatic islets along with defective insulin secretion. We examined IP3-sensitive calcium pools in the brains of Anx7 (+/-) mice by utilizing ATP/Mg 2+-dependent 45Ca 2+ uptake into brain membrane preparations and tissue sections. Although the Anx7 (+/-) mouse brain displayed similar levels of IP3 binding sites and thapsigargin-sensitive 45Ca 2+ uptake as that seen in wild-type mouse brain, the Anx7 (+/-) mouse brain Ca 2+ pools showed markedly reduced sensitivity to IP3. A potent and saturable Ca 2+-releasing effect of recombinant ANX7 protein was demonstrated in mouse and rat brain membrane preparations, which was additive with that of IP3. We propose that ANX7 mobilizes Ca 2+ from an endoplasmic reticulum-like pool, which can be recruited to enhance IP3-mediated Ca 2+ release.

Original languageEnglish
Pages (from-to)151-160
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Issue number1-3
StatePublished - 6 Dec 2004
Externally publishedYes


  • Annexin 7
  • Brain
  • Calcium
  • Endoplasmic reticulum
  • IP3 receptor
  • Knockout mouse
  • Signaling


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