Antibiofilm potential of a negative pressure wound therapy foam loaded with a first-in-class tri-alkyl norspermidine-biaryl antibiotic

Kaden B. Rawson, Travis Neuberger, Tyler Smith, Hariprasada Reddy Kanna Reddy, Travis J. Haussener, Paul R. Sebahar, Ryan E. Looper, Brad M. Isaacson, John Shero, Paul F. Pasquina, Dustin L. Williams*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Negative-pressure wound therapy (NPWT) is commonly utilized to treat traumatic injuries sustained on the modern battlefield. However, NPWT has failed to decrease the incidence of deep tissue infections experienced by Wounded Warriors, despite attempts to integrate common antimicrobials, like Ag+ nanoparticles, into the wound dressing. The purpose of this study was to incorporate a unique antibiofilm compound (CZ-01179) into the polyurethane matrix of NPWT foam via lyophilized hydrogel scaffolding. Foam samples with 2.5%, 5.0%, and 10.0% w/w CZ-01179 were produced and antibiofilm efficacy was compared to the current standards of care: V.A.C.® GRANUFOAM SILVER™ and V.A.C.® GRANUFOAM™. Gravimetric analysis and elution kinetics testing confirmed that this loading technique was both repeatable and controllable. Furthermore, zone of inhibition and antibiofilm efficacy testing showed that foam loaded with CZ-01179 had significantly increased activity against planktonic and biofilm phenotypes of methicillin-resistant Staphylococcus aureus and Acinetobacter baumannii compared to the clinical standards. These findings motivate additional ex vivo and in vivo work with NPWT foam loaded with CZ-01179 with the overall objective of reducing NPWT-associated infections that complicate battlefield-related and other wounds.

Original languageEnglish
Pages (from-to)1780-1788
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume110
Issue number8
DOIs
StatePublished - Aug 2022
Externally publishedYes

Keywords

  • antimicrobial
  • drug delivery/release
  • hydrogel
  • polyurethane(s)
  • wound healing

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