TY - JOUR
T1 - Antibodies induced by liposomal protein exhibit dual binding to protein and lipid epitopes
AU - Karasavvas, Nicos
AU - Beck, Zoltan
AU - Tong, James
AU - Matyas, Gary R.
AU - Rao, Mangala
AU - McCutchan, Francine E.
AU - Michael, Nelson L.
AU - Alving, Carl R.
N1 - Funding Information:
This work was supported through Cooperative Agreement no. DAMD17-93-V-3004 between the Henry M. Jackson Foundation for the Advancement of Military Medicine and the US Army Medical Research and Material Command, working together with the Division of AIDS, National Institute for Allergy and Infectious Diseases, NIH, Bethesda, MD. Research was conducted in compliance with the Animal Welfare Act and other federal statutes and regulations relating to animals and experiments involving animals and adheres to principles stated in the Guide for the Care and Use of Laboratory Animals, NRC Publication, 1996 edition. The views and opinions expressed herein are the private opinions of the authors and do not necessarily reflect the views of the US Army or the US Department of Defense.
PY - 2008/2/22
Y1 - 2008/2/22
N2 - Natural polyreactive antibodies can accommodate chemically unrelated epitopes, such as lipids and proteins, in a single antigen binding site. Because liposomes containing lipid A as an adjuvant can induce antibodies directed against specific lipids, we immunized mice with liposomes containing lipid A together with a protein or peptide antigen to determine whether monoclonal antibodies generated after immunization would be specifically directed both to the liposomal lipid (either cholesterol or galactosylceramide) and also to the accompanying liposomal protein or peptide. Monoclonal antibodies were obtained that bound, by ELISA, to cholesterol and to recombinant gp140 envelope protein from HIV-1, or to galactosylceramide and to an HIV-1 envelope peptide. Surface plasmon resonance studies with the former antibody showed that the liposomal cholesterol and liposomal gp140 each contributed to the overall binding energy of the antibody to liposomes containing cholesterol and protein.
AB - Natural polyreactive antibodies can accommodate chemically unrelated epitopes, such as lipids and proteins, in a single antigen binding site. Because liposomes containing lipid A as an adjuvant can induce antibodies directed against specific lipids, we immunized mice with liposomes containing lipid A together with a protein or peptide antigen to determine whether monoclonal antibodies generated after immunization would be specifically directed both to the liposomal lipid (either cholesterol or galactosylceramide) and also to the accompanying liposomal protein or peptide. Monoclonal antibodies were obtained that bound, by ELISA, to cholesterol and to recombinant gp140 envelope protein from HIV-1, or to galactosylceramide and to an HIV-1 envelope peptide. Surface plasmon resonance studies with the former antibody showed that the liposomal cholesterol and liposomal gp140 each contributed to the overall binding energy of the antibody to liposomes containing cholesterol and protein.
KW - Antibodies to cholesterol
KW - Antibodies to galactosylceramide
KW - Antibody binding specificity
KW - Lipid A
KW - Liposomes
KW - Monoclonal antibodies
KW - Natural antibodies
KW - Polyreactive antibodies
KW - Surface plasmon resonance
UR - http://www.scopus.com/inward/record.url?scp=37549028826&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.12.057
DO - 10.1016/j.bbrc.2007.12.057
M3 - Article
C2 - 18088597
AN - SCOPUS:37549028826
SN - 0006-291X
VL - 366
SP - 982
EP - 987
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -