Antibodies to quinolinic acid reveal localization in select immune cells rather than neurons or astroglia

John R. Moffett; Michael G. Espey; Stephen J. Gaudet; M. A.Aryan Namboodiri

doi:10.1016/0006-8993(93)91450-7

Antibodies to quinolinic acid reveal localization in select immune cells rather than neurons or astroglia

John R. Moffett^*, Michael G. Espey, Stephen J. Gaudet, M. A.Aryan Namboodiri

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Polyclonal antibodies were produced against quinolinic acid. No immunoreactivity was observed in any cell type in carbodiimide-fixed brain tissue from control rats. When the antibodies were applied to carbodiimide-fixed spleen tissue, strong quinolinic acid immunoreactivity was observed in some cells with the appearance of macrophages and dendritic cells. These findings indicate an immune system origin for quinolinic acid, and implicate immune cells in excitotoxic CNS pathologies. These findings also raise the possibility that quinolinic acid is a unique cytokine in immune system signal transmission.

Original language	English
Pages (from-to)	337-340
Number of pages	4
Journal	Brain Research
Volume	623
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(93)91450-7
State	Published - 1 Oct 1993

Keywords

Cytokine
Dendritic cell
Excitotoxin
Immunohistochemistry
Macrophage
Quinolinic acid

Access to Document

10.1016/0006-8993(93)91450-7

Cite this

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title = "Antibodies to quinolinic acid reveal localization in select immune cells rather than neurons or astroglia",

abstract = "Polyclonal antibodies were produced against quinolinic acid. No immunoreactivity was observed in any cell type in carbodiimide-fixed brain tissue from control rats. When the antibodies were applied to carbodiimide-fixed spleen tissue, strong quinolinic acid immunoreactivity was observed in some cells with the appearance of macrophages and dendritic cells. These findings indicate an immune system origin for quinolinic acid, and implicate immune cells in excitotoxic CNS pathologies. These findings also raise the possibility that quinolinic acid is a unique cytokine in immune system signal transmission.",

keywords = "Cytokine, Dendritic cell, Excitotoxin, Immunohistochemistry, Macrophage, Quinolinic acid",

author = "Moffett, {John R.} and Espey, {Michael G.} and Gaudet, {Stephen J.} and Namboodiri, {M. A.Aryan}",

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AU - Moffett, John R.

AU - Espey, Michael G.

AU - Gaudet, Stephen J.

AU - Namboodiri, M. A.Aryan

PY - 1993/10/1

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N2 - Polyclonal antibodies were produced against quinolinic acid. No immunoreactivity was observed in any cell type in carbodiimide-fixed brain tissue from control rats. When the antibodies were applied to carbodiimide-fixed spleen tissue, strong quinolinic acid immunoreactivity was observed in some cells with the appearance of macrophages and dendritic cells. These findings indicate an immune system origin for quinolinic acid, and implicate immune cells in excitotoxic CNS pathologies. These findings also raise the possibility that quinolinic acid is a unique cytokine in immune system signal transmission.

AB - Polyclonal antibodies were produced against quinolinic acid. No immunoreactivity was observed in any cell type in carbodiimide-fixed brain tissue from control rats. When the antibodies were applied to carbodiimide-fixed spleen tissue, strong quinolinic acid immunoreactivity was observed in some cells with the appearance of macrophages and dendritic cells. These findings indicate an immune system origin for quinolinic acid, and implicate immune cells in excitotoxic CNS pathologies. These findings also raise the possibility that quinolinic acid is a unique cytokine in immune system signal transmission.

KW - Cytokine

KW - Dendritic cell

KW - Excitotoxin

KW - Immunohistochemistry

KW - Macrophage

KW - Quinolinic acid

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