Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunization

Yoshihiro Kaku; Akira Noguchi; Glenn A. Marsh; Jennifer A. Barr; Akiko Okutani; Kozue Hotta; Boldbaatar Bazartseren; Christopher C. Broder; Akio Yamada; Satoshi Inoue; Lin Fa Wang

doi:10.1007/s00705-012-1338-3

Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunization

Yoshihiro Kaku^*, Akira Noguchi, Glenn A. Marsh, Jennifer A. Barr, Akiko Okutani, Kozue Hotta, Boldbaatar Bazartseren, Christopher C. Broder, Akio Yamada, Satoshi Inoue, Lin Fa Wang

^*Corresponding author for this work

Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A novel antigen-capture sandwich ELISA system targeting the glycoproteins of the henipaviruses Nipah virus (NiV) and Hendra virus (HeV) was developed. Utilizing purified polyclonal antibodies derived from NiV glycoprotein-encoding DNA-immunized rabbits, we established a system that can detect the native antigenic structures of the henipavirus surface glycoproteins using simplified and inexpensive methods. The lowest detection limit against live viruses was achieved for NiV Bangladesh strain, 2. 5 × 10 ⁴ TCID ₅₀. Considering the recent emergence of genetic variants of henipaviruses and the resultant problems that arise for PCR-based detection, this system could serve as an alternative rapid diagnostic and detection assay.

Original language	English
Pages (from-to)	1605-1609
Number of pages	5
Journal	Archives of Virology
Volume	157
Issue number	8
DOIs	https://doi.org/10.1007/s00705-012-1338-3
State	Published - Aug 2012

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10.1007/s00705-012-1338-3

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title = "Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunization",

abstract = "A novel antigen-capture sandwich ELISA system targeting the glycoproteins of the henipaviruses Nipah virus (NiV) and Hendra virus (HeV) was developed. Utilizing purified polyclonal antibodies derived from NiV glycoprotein-encoding DNA-immunized rabbits, we established a system that can detect the native antigenic structures of the henipavirus surface glycoproteins using simplified and inexpensive methods. The lowest detection limit against live viruses was achieved for NiV Bangladesh strain, 2. 5 × 10 4 TCID 50. Considering the recent emergence of genetic variants of henipaviruses and the resultant problems that arise for PCR-based detection, this system could serve as an alternative rapid diagnostic and detection assay.",

author = "Yoshihiro Kaku and Akira Noguchi and Marsh, {Glenn A.} and Barr, {Jennifer A.} and Akiko Okutani and Kozue Hotta and Boldbaatar Bazartseren and Broder, {Christopher C.} and Akio Yamada and Satoshi Inoue and Wang, {Lin Fa}",

year = "2012",

month = aug,

doi = "10.1007/s00705-012-1338-3",

language = "English",

volume = "157",

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journal = "Archives of Virology",

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T1 - Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunization

AU - Kaku, Yoshihiro

AU - Noguchi, Akira

AU - Marsh, Glenn A.

AU - Barr, Jennifer A.

AU - Okutani, Akiko

AU - Hotta, Kozue

AU - Bazartseren, Boldbaatar

AU - Broder, Christopher C.

AU - Yamada, Akio

AU - Inoue, Satoshi

AU - Wang, Lin Fa

PY - 2012/8

Y1 - 2012/8

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AB - A novel antigen-capture sandwich ELISA system targeting the glycoproteins of the henipaviruses Nipah virus (NiV) and Hendra virus (HeV) was developed. Utilizing purified polyclonal antibodies derived from NiV glycoprotein-encoding DNA-immunized rabbits, we established a system that can detect the native antigenic structures of the henipavirus surface glycoproteins using simplified and inexpensive methods. The lowest detection limit against live viruses was achieved for NiV Bangladesh strain, 2. 5 × 10 4 TCID 50. Considering the recent emergence of genetic variants of henipaviruses and the resultant problems that arise for PCR-based detection, this system could serve as an alternative rapid diagnostic and detection assay.

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