Antimicrobial resistance determinant microarray for analysis of multi-drug resistant isolates

Chris Rowe Taitt; Tomasz Leski; David Stenger; Gary J. Vora; Brent House; Matilda Nicklasson; Guillermo Pimentel; Daniel V. Zurawski; Benjamin C. Kirkup; David Craft; Paige E. Waterman; Emil P. Lesho; Umaru Bangura; Rashid Ansumana

doi:10.1117/12.924569

Antimicrobial resistance determinant microarray for analysis of multi-drug resistant isolates

Chris Rowe Taitt^*, Tomasz Leski, David Stenger, Gary J. Vora, Brent House, Matilda Nicklasson, Guillermo Pimentel, Daniel V. Zurawski, Benjamin C. Kirkup, David Craft, Paige E. Waterman, Emil P. Lesho, Umaru Bangura, Rashid Ansumana

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Scopus citations

Abstract

The prevalence of multidrug-resistant infections in personnel wounded in Iraq and Afghanistan has made it challenging for physicians to choose effective therapeutics in a timely fashion. To address the challenge of identifying the potential for drug resistance, we have developed the Antimicrobial Resistance Determinant Microarray (ARDM) to provide DNAbased analysis for over 250 resistance genes covering 12 classes of antibiotics. Over 70 drug-resistant bacteria from different geographic regions have been analyzed on ARDM, with significant differences in patterns of resistance identified: genes for resistance to sulfonamides, trimethoprim, chloramphenicol, rifampin, and macrolide-lincosamidesulfonamide drugs were more frequently identified in isolates from sources in Iraq/Afghanistan. Of particular concern was the presence of genes responsible for resistance to many of the last-resort antibiotics used to treat war traumaassociated infections.

Original language	English
Title of host publication	Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX
DOIs	https://doi.org/10.1117/12.924569
State	Published - 2012
Externally published	Yes
Event	Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX - Baltimore, MD, United States Duration: 23 Apr 2012 → 25 Apr 2012

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	8371
ISSN (Print)	0277-786X

Conference

Conference	Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX
Country/Territory	United States
City	Baltimore, MD
Period	23/04/12 → 25/04/12

Keywords

Acinetobacter
Antimicrobial resistance
E. coli
Klebsiella
Microarray

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10.1117/12.924569

Cite this

Taitt, C. R., Leski, T., Stenger, D., Vora, G. J., House, B., Nicklasson, M., Pimentel, G., Zurawski, D. V., Kirkup, B. C., Craft, D., Waterman, P. E., Lesho, E. P., Bangura, U., & Ansumana, R. (2012). Antimicrobial resistance determinant microarray for analysis of multi-drug resistant isolates. In Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX Article 83710X (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8371). https://doi.org/10.1117/12.924569

Taitt, Chris Rowe ; Leski, Tomasz ; Stenger, David et al. / Antimicrobial resistance determinant microarray for analysis of multi-drug resistant isolates. Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX. 2012. (Proceedings of SPIE - The International Society for Optical Engineering).

@inproceedings{42350050fc8b4062b1b66c41b9722f58,

title = "Antimicrobial resistance determinant microarray for analysis of multi-drug resistant isolates",

abstract = "The prevalence of multidrug-resistant infections in personnel wounded in Iraq and Afghanistan has made it challenging for physicians to choose effective therapeutics in a timely fashion. To address the challenge of identifying the potential for drug resistance, we have developed the Antimicrobial Resistance Determinant Microarray (ARDM) to provide DNAbased analysis for over 250 resistance genes covering 12 classes of antibiotics. Over 70 drug-resistant bacteria from different geographic regions have been analyzed on ARDM, with significant differences in patterns of resistance identified: genes for resistance to sulfonamides, trimethoprim, chloramphenicol, rifampin, and macrolide-lincosamidesulfonamide drugs were more frequently identified in isolates from sources in Iraq/Afghanistan. Of particular concern was the presence of genes responsible for resistance to many of the last-resort antibiotics used to treat war traumaassociated infections.",

keywords = "Acinetobacter, Antimicrobial resistance, E. coli, Klebsiella, Microarray",

author = "Taitt, {Chris Rowe} and Tomasz Leski and David Stenger and Vora, {Gary J.} and Brent House and Matilda Nicklasson and Guillermo Pimentel and Zurawski, {Daniel V.} and Kirkup, {Benjamin C.} and David Craft and Waterman, {Paige E.} and Lesho, {Emil P.} and Umaru Bangura and Rashid Ansumana",

year = "2012",

doi = "10.1117/12.924569",

language = "English",

isbn = "9780819490490",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

booktitle = "Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX",

note = "Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX ; Conference date: 23-04-2012 Through 25-04-2012",

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Taitt, CR, Leski, T, Stenger, D, Vora, GJ, House, B, Nicklasson, M, Pimentel, G, Zurawski, DV, Kirkup, BC, Craft, D, Waterman, PE, Lesho, EP, Bangura, U & Ansumana, R 2012, Antimicrobial resistance determinant microarray for analysis of multi-drug resistant isolates. in Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX., 83710X, Proceedings of SPIE - The International Society for Optical Engineering, vol. 8371, Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX, Baltimore, MD, United States, 23/04/12. https://doi.org/10.1117/12.924569

Antimicrobial resistance determinant microarray for analysis of multi-drug resistant isolates. / Taitt, Chris Rowe; Leski, Tomasz; Stenger, David et al.
Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX. 2012. 83710X (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8371).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Antimicrobial resistance determinant microarray for analysis of multi-drug resistant isolates

AU - Taitt, Chris Rowe

AU - Leski, Tomasz

AU - Stenger, David

AU - Vora, Gary J.

AU - House, Brent

AU - Nicklasson, Matilda

AU - Pimentel, Guillermo

AU - Zurawski, Daniel V.

AU - Kirkup, Benjamin C.

AU - Craft, David

AU - Waterman, Paige E.

AU - Lesho, Emil P.

AU - Bangura, Umaru

AU - Ansumana, Rashid

PY - 2012

Y1 - 2012

N2 - The prevalence of multidrug-resistant infections in personnel wounded in Iraq and Afghanistan has made it challenging for physicians to choose effective therapeutics in a timely fashion. To address the challenge of identifying the potential for drug resistance, we have developed the Antimicrobial Resistance Determinant Microarray (ARDM) to provide DNAbased analysis for over 250 resistance genes covering 12 classes of antibiotics. Over 70 drug-resistant bacteria from different geographic regions have been analyzed on ARDM, with significant differences in patterns of resistance identified: genes for resistance to sulfonamides, trimethoprim, chloramphenicol, rifampin, and macrolide-lincosamidesulfonamide drugs were more frequently identified in isolates from sources in Iraq/Afghanistan. Of particular concern was the presence of genes responsible for resistance to many of the last-resort antibiotics used to treat war traumaassociated infections.

AB - The prevalence of multidrug-resistant infections in personnel wounded in Iraq and Afghanistan has made it challenging for physicians to choose effective therapeutics in a timely fashion. To address the challenge of identifying the potential for drug resistance, we have developed the Antimicrobial Resistance Determinant Microarray (ARDM) to provide DNAbased analysis for over 250 resistance genes covering 12 classes of antibiotics. Over 70 drug-resistant bacteria from different geographic regions have been analyzed on ARDM, with significant differences in patterns of resistance identified: genes for resistance to sulfonamides, trimethoprim, chloramphenicol, rifampin, and macrolide-lincosamidesulfonamide drugs were more frequently identified in isolates from sources in Iraq/Afghanistan. Of particular concern was the presence of genes responsible for resistance to many of the last-resort antibiotics used to treat war traumaassociated infections.

KW - Acinetobacter

KW - Antimicrobial resistance

KW - E. coli

KW - Klebsiella

KW - Microarray

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DO - 10.1117/12.924569

M3 - Conference contribution

AN - SCOPUS:84863887534

SN - 9780819490490

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX

T2 - Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX

Y2 - 23 April 2012 through 25 April 2012

ER -

Taitt CR, Leski T, Stenger D, Vora GJ, House B, Nicklasson M et al. Antimicrobial resistance determinant microarray for analysis of multi-drug resistant isolates. In Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring II; and Biometric Technology for Human Identification IX. 2012. 83710X. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.924569

Antimicrobial resistance determinant microarray for analysis of multi-drug resistant isolates

Abstract

Publication series

Conference

Keywords

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