Antimicrobial susceptibilities of geographically diverse clinical human isolates of Leptospira

Roseanne A. Ressner, Matthew E. Griffith, Miriam L. Beckius, Guillermo Pimentel, R. Scott Miller, Katrin Mende, Susan L. Fraser, Renee L. Galloway, Duane R. Hospenthal, Clinton K. Murray

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Although antimicrobial therapy of leptospirosis has been studied in a few randomized controlled clinical studies, those studies were limited to specific regions of the world and few have characterized infecting strains. A broth microdilution technique for the assessment of antibiotic susceptibility has been developed at Brooke Army Medical Center. In the present study, we assessed the susceptibilities of 13 Leptospira isolates (including recent clinical isolates) from Egypt, Thailand, Nicaragua, and Hawaii to 13 antimicrobial agents. Ampicillin, cefepime, azithromycin, and clarithromycin were found to have MICs below the lower limit of detection (0.016 μg/ml). Cefotaxime, ceftriaxone, imipenem-cilastatin, penicillin G, moxifloxacin, ciprofloxacin, and levofloxacin had MIC90S between 0.030 and 0.125 μg/ml. Doxycycline and tetracycline had the highest MIC90S: 2 and 4 μg/ml, respectively. Doxycycline and tetracycline were noted to have slightly higher MICs against isolates from Egypt than against strains from Thailand or Hawaii; otherwise, the susceptibility patterns were similar. There appears to be possible variability in susceptibility to some antimicrobial agents among strains, suggesting that more extensive testing to look for geographic variability should be pursued.

Original languageEnglish
Pages (from-to)2750-2754
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume52
Issue number8
DOIs
StatePublished - Aug 2008
Externally publishedYes

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