Anx7, a candidate tumor suppressor gene for prostate cancer

Meera Srivastava*, Lukas Bubendorf, Vasantha Srikantan, Linda Fossom, Lisa Nolan, Mirta Glasman, Ximena Leighton, Wilfred Fehrle, Stefania Pittaluga, Mark Raffeld, Pasi Koivisto, Niels Willi, Thomas C. Gasser, Juha Kononen, Guido Sauter, Olli P. Kallioniemi, Shiv Srivastava, Harvey B. Pollard

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

127 Scopus citations


The ANX7 gene is located on human chromosome 10q21, a site long hypothesized to harbor a tumor suppressor gene(s) (TSG) associated with prostate and other cancers. To test whether ANX7 might be a candidate TSG, we examined the ANX7-dependent suppression of human tumor cell growth, stage-specific ANX7 expression in 301 prostate specimens on a prostate tissue microarray, and loss of heterozygosity (LOH) of microsatellite markers at or near the ANX7 locus. Here we report that human tumor cell proliferation and colony formation are markedly reduced when the wild-type ANX7 gene is transfected into two prostate tumor cell lines, LNCaP and DU145. Consistently, analysis of ANX7 protein expression in human prostate tumor microarrays reveals a significantly higher rate of loss of ANX7 expression in metastatic and local recurrences of hormone refractory prostate cancer as compared with primary tumors (P = 0.0001). Using four microsatellite markers at or near the ANX7 locus, and laser capture microdissected tumor cells, 35% of the 20 primary prostate tumors show LOH. The microsatellite marker closest to the ANX7 locus showed the highest rate of LOH, including one homozygous deletion. We conclude that the ANX7 gene exhibits many biological and genetic properties expected of a TSG and may play a role in prostate cancer progression.

Original languageEnglish
Pages (from-to)4575-4580
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number8
StatePublished - 10 Apr 2001
Externally publishedYes


  • Cancer genetics
  • Chromosome 10q21
  • Loss of heterozygosity


Dive into the research topics of 'Anx7, a candidate tumor suppressor gene for prostate cancer'. Together they form a unique fingerprint.

Cite this