Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery

Weidong Zhang, Rajeswari Singam, Gary Hellermann, Xiaoyuan Kong, Homero San Juan, Richard F. Lockey, Shuen Ju Wu, Kevin Porter, Shyam S. Mohapatra*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Background: The need for safe and effective treatment of dengue virus (DEN), a class A agent that causes dengue hemorrhagic fever/dengue shock syndrome, has been a critical global priority. An effective vaccine for DEN is not yet available. In this study the possibility of attenuating DEN infection using adeno-associated virus (AAV)-encoded short interfering RNAs (siRNA) was examined in Vero cells and human dendritic cells (DCs). Methods: A cassette encoding siRNA targeted to a 3′ untranslated sequence common to all DEN serotypes was designed and tested for its ability to attenuate DEN infection by use of AAV delivery. Results: Vero cells or DCs infected with AAV-siRNA showed a significant, dose-dependent reduction in DEN infection. Treatment of DCs with AAV-siRNA also decreased the DEN-induced apoptosis of DCs and did not induce significant inflammation. Conclusion: These results demonstrate that AAV-mediated siRNA delivery is capable of reducing DEN infection in cells and may be useful in decreasing DEN replication in humans.

Original languageEnglish
JournalGenetic Vaccines and Therapy
StatePublished - 9 Aug 2004
Externally publishedYes


  • Adeno-associated virus
  • Dengue virus
  • Gene expression
  • siRNA


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