Autofluorescence of Toxoplasma gondii and related coccidian oocysts

H. D.Alan Lindquist; Jason W. Bennett; Jeff D. Hester; Michael W. Ware; J. P. Dubey; William V. Everson

doi:10.1645/GE-3147RN

Autofluorescence of Toxoplasma gondii and related coccidian oocysts

H. D.Alan Lindquist^*, Jason W. Bennett, Jeff D. Hester, Michael W. Ware, J. P. Dubey, William V. Everson

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

This is the first report of blue autofluorescence as a useful characteristic in the microscopic detection of Toxoplasma gondii, Hammondia hammondi, Hammondia heydorni, Neospora caninum, Besnoitia darlingi, and Sarcocystis neurona oocysts or sporocysts. This autofluorescence is of sufficient intensity and duration to allow identification of these oocysts from complex microscopic sample backgrounds. As with the autofluorescence of related coccidia, the oocysts glow pale blue when illuminated with an ultraviolet (UV) light source and viewed with the correct UV excitation and emission filter set.

Original language	English
Pages (from-to)	865-867
Number of pages	3
Journal	Journal of Parasitology
Volume	89
Issue number	4
DOIs	https://doi.org/10.1645/GE-3147RN
State	Published - Aug 2003
Externally published	Yes

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10.1645/GE-3147RN

Cite this

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title = "Autofluorescence of Toxoplasma gondii and related coccidian oocysts",

abstract = "This is the first report of blue autofluorescence as a useful characteristic in the microscopic detection of Toxoplasma gondii, Hammondia hammondi, Hammondia heydorni, Neospora caninum, Besnoitia darlingi, and Sarcocystis neurona oocysts or sporocysts. This autofluorescence is of sufficient intensity and duration to allow identification of these oocysts from complex microscopic sample backgrounds. As with the autofluorescence of related coccidia, the oocysts glow pale blue when illuminated with an ultraviolet (UV) light source and viewed with the correct UV excitation and emission filter set.",

author = "Lindquist, {H. D.Alan} and Bennett, {Jason W.} and Hester, {Jeff D.} and Ware, {Michael W.} and Dubey, {J. P.} and Everson, {William V.}",

year = "2003",

month = aug,

doi = "10.1645/GE-3147RN",

language = "English",

volume = "89",

pages = "865--867",

journal = "Journal of Parasitology",

issn = "0022-3395",

number = "4",

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T1 - Autofluorescence of Toxoplasma gondii and related coccidian oocysts

AU - Lindquist, H. D.Alan

AU - Bennett, Jason W.

AU - Hester, Jeff D.

AU - Ware, Michael W.

AU - Dubey, J. P.

AU - Everson, William V.

PY - 2003/8

Y1 - 2003/8

N2 - This is the first report of blue autofluorescence as a useful characteristic in the microscopic detection of Toxoplasma gondii, Hammondia hammondi, Hammondia heydorni, Neospora caninum, Besnoitia darlingi, and Sarcocystis neurona oocysts or sporocysts. This autofluorescence is of sufficient intensity and duration to allow identification of these oocysts from complex microscopic sample backgrounds. As with the autofluorescence of related coccidia, the oocysts glow pale blue when illuminated with an ultraviolet (UV) light source and viewed with the correct UV excitation and emission filter set.

AB - This is the first report of blue autofluorescence as a useful characteristic in the microscopic detection of Toxoplasma gondii, Hammondia hammondi, Hammondia heydorni, Neospora caninum, Besnoitia darlingi, and Sarcocystis neurona oocysts or sporocysts. This autofluorescence is of sufficient intensity and duration to allow identification of these oocysts from complex microscopic sample backgrounds. As with the autofluorescence of related coccidia, the oocysts glow pale blue when illuminated with an ultraviolet (UV) light source and viewed with the correct UV excitation and emission filter set.

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SN - 0022-3395

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EP - 867

JO - Journal of Parasitology

JF - Journal of Parasitology

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