Autoimmune Profiling Reveals Peroxiredoxin 6 as a Candidate Traumatic Brain Injury Biomarker

John E. Buonora; Michael Mousseau; David M. Jacobowitz; Rachel C. Lazarus; Angela M. Yarnell; Cara H. Olsen; Harvey B. Pollard; Ramon Diaz-Arrastia; Lawrence Latour; Gregory P. Mueller

doi:10.1089/neu.2014.3736

Autoimmune Profiling Reveals Peroxiredoxin 6 as a Candidate Traumatic Brain Injury Biomarker

John E. Buonora, Michael Mousseau, David M. Jacobowitz, Rachel C. Lazarus, Angela M. Yarnell, Cara H. Olsen, Harvey B. Pollard, Ramon Diaz-Arrastia, Lawrence Latour, Gregory P. Mueller^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Autoimmune profiling in rats revealed the antioxidant enzyme, peroxiredoxin 6 (PRDX6), as a target for autoantibodies evoked in response to traumatic brain injury (TBI). Consistent with this proposal, immunohistochemical analysis of rat cerebral cortex demonstrated that PRDX6 is highly expressed in the perivascular space, presumably contained within astrocytic foot processes. Accordingly, an immunosorbent electrochemiluminescence assay was developed for investigating PRDX6 in human samples. PRDX6 was found to be measurable in human blood and highly expressed in human cerebral cortex and platelets. Circulating levels of PRDX6 were elevated fourfold over control values 4 to 24 h following mild-to-moderate TBI. These findings suggest that PRDX6 may serve as a biomarker for TBI and that autoimmune profiling is a viable strategy for the discovery of novel TBI biomarkers.

Original language	English
Pages (from-to)	1805-1814
Number of pages	10
Journal	Journal of Neurotrauma
Volume	32
Issue number	22
DOIs	https://doi.org/10.1089/neu.2014.3736
State	Published - 15 Nov 2015
Externally published	Yes

Keywords

autoantibodies
autoimmune profiling
biomarker
peroxiredoxin 6
traumatic brain injury

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title = "Autoimmune Profiling Reveals Peroxiredoxin 6 as a Candidate Traumatic Brain Injury Biomarker",

abstract = "Autoimmune profiling in rats revealed the antioxidant enzyme, peroxiredoxin 6 (PRDX6), as a target for autoantibodies evoked in response to traumatic brain injury (TBI). Consistent with this proposal, immunohistochemical analysis of rat cerebral cortex demonstrated that PRDX6 is highly expressed in the perivascular space, presumably contained within astrocytic foot processes. Accordingly, an immunosorbent electrochemiluminescence assay was developed for investigating PRDX6 in human samples. PRDX6 was found to be measurable in human blood and highly expressed in human cerebral cortex and platelets. Circulating levels of PRDX6 were elevated fourfold over control values 4 to 24 h following mild-to-moderate TBI. These findings suggest that PRDX6 may serve as a biomarker for TBI and that autoimmune profiling is a viable strategy for the discovery of novel TBI biomarkers.",

keywords = "autoantibodies, autoimmune profiling, biomarker, peroxiredoxin 6, traumatic brain injury",

author = "Buonora, {John E.} and Michael Mousseau and Jacobowitz, {David M.} and Lazarus, {Rachel C.} and Yarnell, {Angela M.} and Olsen, {Cara H.} and Pollard, {Harvey B.} and Ramon Diaz-Arrastia and Lawrence Latour and Mueller, {Gregory P.}",

note = "Publisher Copyright: {\textcopyright} Mary Ann Liebert, Inc. 2015.",

year = "2015",

month = nov,

day = "15",

doi = "10.1089/neu.2014.3736",

language = "English",

volume = "32",

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journal = "Journal of Neurotrauma",

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T1 - Autoimmune Profiling Reveals Peroxiredoxin 6 as a Candidate Traumatic Brain Injury Biomarker

AU - Buonora, John E.

AU - Mousseau, Michael

AU - Jacobowitz, David M.

AU - Lazarus, Rachel C.

AU - Yarnell, Angela M.

AU - Olsen, Cara H.

AU - Pollard, Harvey B.

AU - Diaz-Arrastia, Ramon

AU - Latour, Lawrence

AU - Mueller, Gregory P.

N1 - Publisher Copyright: © Mary Ann Liebert, Inc. 2015.

PY - 2015/11/15

Y1 - 2015/11/15

N2 - Autoimmune profiling in rats revealed the antioxidant enzyme, peroxiredoxin 6 (PRDX6), as a target for autoantibodies evoked in response to traumatic brain injury (TBI). Consistent with this proposal, immunohistochemical analysis of rat cerebral cortex demonstrated that PRDX6 is highly expressed in the perivascular space, presumably contained within astrocytic foot processes. Accordingly, an immunosorbent electrochemiluminescence assay was developed for investigating PRDX6 in human samples. PRDX6 was found to be measurable in human blood and highly expressed in human cerebral cortex and platelets. Circulating levels of PRDX6 were elevated fourfold over control values 4 to 24 h following mild-to-moderate TBI. These findings suggest that PRDX6 may serve as a biomarker for TBI and that autoimmune profiling is a viable strategy for the discovery of novel TBI biomarkers.

AB - Autoimmune profiling in rats revealed the antioxidant enzyme, peroxiredoxin 6 (PRDX6), as a target for autoantibodies evoked in response to traumatic brain injury (TBI). Consistent with this proposal, immunohistochemical analysis of rat cerebral cortex demonstrated that PRDX6 is highly expressed in the perivascular space, presumably contained within astrocytic foot processes. Accordingly, an immunosorbent electrochemiluminescence assay was developed for investigating PRDX6 in human samples. PRDX6 was found to be measurable in human blood and highly expressed in human cerebral cortex and platelets. Circulating levels of PRDX6 were elevated fourfold over control values 4 to 24 h following mild-to-moderate TBI. These findings suggest that PRDX6 may serve as a biomarker for TBI and that autoimmune profiling is a viable strategy for the discovery of novel TBI biomarkers.

KW - autoantibodies

KW - autoimmune profiling

KW - biomarker

KW - peroxiredoxin 6

KW - traumatic brain injury

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JO - Journal of Neurotrauma

JF - Journal of Neurotrauma

IS - 22

ER -

Autoimmune Profiling Reveals Peroxiredoxin 6 as a Candidate Traumatic Brain Injury Biomarker

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Keywords

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