TY - JOUR
T1 - B-cell subsets responsive to fluorescein-conjugated antigens
T2 - II. “Cross-priming” and its elimination by bromodeoxyuridine and light
AU - Scott, David W.
AU - Alexander, Christian
N1 - Funding Information:
’ This work was supported by U.S. Public Health Service Grant AI-10716. * D.W.S. is a recipient of a Research Career Development Award, Grant AI-00093. 3 Abbreviations used: BrA, Bruce/la abortus; FL, fluorescein hapten; FITC, fluorescein isothiocyanate; LPS, lipopolysaccharide; PFC, plaque-forming cells; PBAr, polyclonal B-cell-activating receptors; POL, polymerized flagellin; PPD, purified protein derivative; RBC, red blood cells; TD, T-cell dependent; TI, T-cell independent.
PY - 1980
Y1 - 1980
N2 - In vitro stimulation of normal splenic B cells with a variety of fluorescein (FL)-conjugated immunogens stimulated the incorporation of bromodeoxyuridine to the extent that all subsequent FL-immunogen-induced PFC responses were inhibited after exposure to light. The effect was hapten specific and suggests that any FL-immunogen can trigger an initial wave of DNA synthesis independent of surface IgD or polyclonal B-cell activating receptor interaction.
AB - In vitro stimulation of normal splenic B cells with a variety of fluorescein (FL)-conjugated immunogens stimulated the incorporation of bromodeoxyuridine to the extent that all subsequent FL-immunogen-induced PFC responses were inhibited after exposure to light. The effect was hapten specific and suggests that any FL-immunogen can trigger an initial wave of DNA synthesis independent of surface IgD or polyclonal B-cell activating receptor interaction.
UR - http://www.scopus.com/inward/record.url?scp=0018959254&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(80)90337-8
DO - 10.1016/0008-8749(80)90337-8
M3 - Article
C2 - 6967767
AN - SCOPUS:0018959254
SN - 0008-8749
VL - 53
SP - 376
EP - 381
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -