B-cell subsets responsive to fluorescein-conjugated antigens: II. “Cross-priming” and its elimination by bromodeoxyuridine and light

In vitro stimulation of normal splenic B cells with a variety of fluorescein (FL)-conjugated immunogens stimulated the incorporation of bromodeoxyuridine to the extent that all subsequent FL-immunogen-induced PFC responses were inhibited after exposure to light. The effect was hapten specific and suggests that any FL-immunogen can trigger an initial wave of DNA synthesis independent of surface IgD or polyclonal B-cell activating receptor interaction.