TY - JOUR
T1 - B-cell subsets responsive to fluorescein-conjugated antigens. IV. Cross-stimulation of B cells genetically unresponsive to LPS by hapten-conjugated lipopolysaccharide
AU - Scott, David W.
AU - Tuttle, Jane
AU - Piper, Margaret
N1 - Funding Information:
’ Supported by National Institutes of Health Grant AI-10716. ’ D.W.S. was supported by Research Career Development Award from the National Institutes of Health, Grant AI-00093.
PY - 1981/7/1
Y1 - 1981/7/1
N2 - B-cell subsets specific for the same hapten share immunoglobulin (Ig) receptors for hapten, but presumably possess different receptors for mitogen. However, we and others recently presented evidence that B cells responsive to different forms of the same hapten (FL) could be activated to clonal expansion by a single FL-antigen, an effect called "cross-priming." Since it was critical to establish that this phenomenon was independent of putative mitogen receptors for a given FL-antigen, we attempted cross-priming of C3H/HeJ spleen cells with FL-conjugated LPS, an antigen to which they are unable to respond by either mitosis or polyclonal differentiation. Our data indicate that FL-LPS does indeed cross-prime C3H/HeJ B cells such that the subsequent response to other FL-antigens is increased. Augmentation of the anti-FL response was elicited by even nanogram doses of FL-LPS, whereas unconjugated LPS (up to 10 μg/ml) was without effect. These data suggest that induction of a positive signal in FL-responsive cells does not require an interaction with putative mitogen receptors but can occur via hapten:Ig receptor interactions.
AB - B-cell subsets specific for the same hapten share immunoglobulin (Ig) receptors for hapten, but presumably possess different receptors for mitogen. However, we and others recently presented evidence that B cells responsive to different forms of the same hapten (FL) could be activated to clonal expansion by a single FL-antigen, an effect called "cross-priming." Since it was critical to establish that this phenomenon was independent of putative mitogen receptors for a given FL-antigen, we attempted cross-priming of C3H/HeJ spleen cells with FL-conjugated LPS, an antigen to which they are unable to respond by either mitosis or polyclonal differentiation. Our data indicate that FL-LPS does indeed cross-prime C3H/HeJ B cells such that the subsequent response to other FL-antigens is increased. Augmentation of the anti-FL response was elicited by even nanogram doses of FL-LPS, whereas unconjugated LPS (up to 10 μg/ml) was without effect. These data suggest that induction of a positive signal in FL-responsive cells does not require an interaction with putative mitogen receptors but can occur via hapten:Ig receptor interactions.
UR - http://www.scopus.com/inward/record.url?scp=0019452675&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(81)90377-4
DO - 10.1016/0008-8749(81)90377-4
M3 - Article
C2 - 6972809
AN - SCOPUS:0019452675
SN - 0008-8749
VL - 61
SP - 292
EP - 299
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -