B cells "transduced" with TAT-fusion proteins can induce tolerance and protect mice from diabetes and EAE

Yan Su, Ai Hong Zhang, Xin Li, Nana Owusu-Boaitey, Jonathan Skupsky, David W. Scott*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Antigen-immunoglobulin fusion protein expressing B cells have been shown as excellent tolerogenic antigen-presenting cells in multiple disease models. Using efficient protein transduction by fusion with a HIV TAT protein transduction domain, we herein tested the TAT-fusion protein transduced B cells for their effects in antigen-specific tolerance induction in two animal models, experimental autoimmune encephalomyelitis (EAE) and type 1 diabetes. We demonstrated that transfer of TAT-MOG35-55 (myelin oligodendrocyte glycoprotein)-Ig 'transduced B cells' 10days after EAE induction significantly protected mice from disease. Similarly, the onset of disease was delayed when NOD mice received insulin specific TAT-B9-23-B cells. Surprisingly, no protection against EAE was observed in a prophylactic protocol when transduced B cells were given before disease induction. Moreover, TAT-ovalbumin transduced cells were tolerogenic in primed but not naïve mice. Our results suggest that TAT-fusion protein transduced B cells were tolerogenic in antigen primed recipients, a condition clinically relevant to autoimmune diseases.

Original languageEnglish
Pages (from-to)260-267
Number of pages8
JournalClinical Immunology
Issue number3
StatePublished - Sep 2011
Externally publishedYes


  • B cells
  • EAE
  • Protein therapy
  • T1D
  • TAT fusion
  • Tolerance


Dive into the research topics of 'B cells "transduced" with TAT-fusion proteins can induce tolerance and protect mice from diabetes and EAE'. Together they form a unique fingerprint.

Cite this