Brain oxygenation with a non-vasoactive perfluorocarbon emulsion in a rat model of traumatic brain injury

Rania Abutarboush*, Saad H. Mullah, Biswajit K. Saha, Ashraful Haque, Peter B. Walker, Chioma Aligbe, Georgina Pappas, Lam Thuy Vi Tran Ho, Francoise G. Arnaud, Charles R. Auker, Richard M. McCarron, Anke H. Scultetus, Paula Moon-Massat

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Objective: The aim of this study was to assess, in two experiments, the safety and efficacy of the PFC emulsion Oxycyte as an oxygen therapeutic for TBI to test the hypothesis that early administration of this oxygen-carrying fluid post-TBI would improve brain tissue oxygenation (PbtO2). Methods: The first experiment assessed the effects of Oxycyte on cerebral vasoactivity in healthy, uninjured rats using intravital microscopy. The second experiment investigated the effect of Oxycyte on cerebral PbtO2 using the PQM in TBI model. Animals in the Oxycyte group received a single injection of Oxycyte (6 mL/kg) shortly after TBI, while NON animals received no treatment. Results: Oxycyte did not cause vasoconstriction in small- (<50 μm) or medium- (50-100 μm) sized pial arterioles nor did it cause a significant change in blood pressure. Treatment with Oxycyte while breathing 100% O2 did not improve PbtO2. However, in rats ventilated with ~40% O2, PbtO2 improved to near pre-TBI values within 105 minutes after Oxycyte injection. Conclusions: Although Oxycyte did not cause cerebral vasoconstriction, its use at the dose tested while breathing 100% O2 did not improve PbtO2 following TBI. However, Oxycyte treatment while breathing a lower enriched oxygen concentration may improve PbtO2 after TBI.

Original languageEnglish
Article numbere12441
Issue number3
StatePublished - Apr 2018
Externally publishedYes


  • controlled cortical impact
  • intravital microscopy
  • oxygen therapeutic
  • phosphorescence quenching method
  • pial microcirculation


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