Brain oxygenation with a non-vasoactive perfluorocarbon emulsion in a rat model of traumatic brain injury

Rania Abutarboush; Saad H. Mullah; Biswajit K. Saha; Ashraful Haque; Peter B. Walker; Chioma Aligbe; Georgina Pappas; Lam Thuy Vi Tran Ho; Francoise G. Arnaud; Charles R. Auker; Richard M. McCarron; Anke H. Scultetus; Paula Moon-Massat

doi:10.1111/micc.12441

Brain oxygenation with a non-vasoactive perfluorocarbon emulsion in a rat model of traumatic brain injury

Rania Abutarboush^*, Saad H. Mullah, Biswajit K. Saha, Ashraful Haque, Peter B. Walker, Chioma Aligbe, Georgina Pappas, Lam Thuy Vi Tran Ho, Francoise G. Arnaud, Charles R. Auker, Richard M. McCarron, Anke H. Scultetus, Paula Moon-Massat

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Objective: The aim of this study was to assess, in two experiments, the safety and efficacy of the PFC emulsion Oxycyte as an oxygen therapeutic for TBI to test the hypothesis that early administration of this oxygen-carrying fluid post-TBI would improve brain tissue oxygenation (P_btO₂). Methods: The first experiment assessed the effects of Oxycyte on cerebral vasoactivity in healthy, uninjured rats using intravital microscopy. The second experiment investigated the effect of Oxycyte on cerebral P_btO₂ using the PQM in TBI model. Animals in the Oxycyte group received a single injection of Oxycyte (6 mL/kg) shortly after TBI, while NON animals received no treatment. Results: Oxycyte did not cause vasoconstriction in small- (<50 μm) or medium- (50-100 μm) sized pial arterioles nor did it cause a significant change in blood pressure. Treatment with Oxycyte while breathing 100% O₂ did not improve P_btO₂. However, in rats ventilated with ~40% O₂, P_btO₂ improved to near pre-TBI values within 105 minutes after Oxycyte injection. Conclusions: Although Oxycyte did not cause cerebral vasoconstriction, its use at the dose tested while breathing 100% O₂ did not improve P_btO₂ following TBI. However, Oxycyte treatment while breathing a lower enriched oxygen concentration may improve P_btO₂ after TBI.

Original language	English
Article number	e12441
Journal	Microcirculation
Volume	25
Issue number	3
DOIs	https://doi.org/10.1111/micc.12441
State	Published - Apr 2018
Externally published	Yes

Keywords

controlled cortical impact
intravital microscopy
oxygen therapeutic
phosphorescence quenching method
pial microcirculation

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10.1111/micc.12441

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Abutarboush, R., Mullah, S. H., Saha, B. K., Haque, A., Walker, P. B., Aligbe, C., Pappas, G., Tran Ho, L. T. V., Arnaud, F. G., Auker, C. R., McCarron, R. M., Scultetus, A. H., & Moon-Massat, P. (2018). Brain oxygenation with a non-vasoactive perfluorocarbon emulsion in a rat model of traumatic brain injury. Microcirculation, 25(3), Article e12441. https://doi.org/10.1111/micc.12441

@article{662c30255ec34d7fba09caabbc536878,

title = "Brain oxygenation with a non-vasoactive perfluorocarbon emulsion in a rat model of traumatic brain injury",

abstract = "Objective: The aim of this study was to assess, in two experiments, the safety and efficacy of the PFC emulsion Oxycyte as an oxygen therapeutic for TBI to test the hypothesis that early administration of this oxygen-carrying fluid post-TBI would improve brain tissue oxygenation (PbtO2). Methods: The first experiment assessed the effects of Oxycyte on cerebral vasoactivity in healthy, uninjured rats using intravital microscopy. The second experiment investigated the effect of Oxycyte on cerebral PbtO2 using the PQM in TBI model. Animals in the Oxycyte group received a single injection of Oxycyte (6 mL/kg) shortly after TBI, while NON animals received no treatment. Results: Oxycyte did not cause vasoconstriction in small- (<50 μm) or medium- (50-100 μm) sized pial arterioles nor did it cause a significant change in blood pressure. Treatment with Oxycyte while breathing 100% O2 did not improve PbtO2. However, in rats ventilated with ~40% O2, PbtO2 improved to near pre-TBI values within 105 minutes after Oxycyte injection. Conclusions: Although Oxycyte did not cause cerebral vasoconstriction, its use at the dose tested while breathing 100% O2 did not improve PbtO2 following TBI. However, Oxycyte treatment while breathing a lower enriched oxygen concentration may improve PbtO2 after TBI.",

keywords = "controlled cortical impact, intravital microscopy, oxygen therapeutic, phosphorescence quenching method, pial microcirculation",

author = "Rania Abutarboush and Mullah, {Saad H.} and Saha, {Biswajit K.} and Ashraful Haque and Walker, {Peter B.} and Chioma Aligbe and Georgina Pappas and {Tran Ho}, {Lam Thuy Vi} and Arnaud, {Francoise G.} and Auker, {Charles R.} and McCarron, {Richard M.} and Scultetus, {Anke H.} and Paula Moon-Massat",

note = "Publisher Copyright: {\textcopyright} 2018 John Wiley & Sons Ltd",

year = "2018",

month = apr,

doi = "10.1111/micc.12441",

language = "English",

volume = "25",

journal = "Microcirculation",

issn = "1073-9688",

number = "3",

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T1 - Brain oxygenation with a non-vasoactive perfluorocarbon emulsion in a rat model of traumatic brain injury

AU - Abutarboush, Rania

AU - Mullah, Saad H.

AU - Saha, Biswajit K.

AU - Haque, Ashraful

AU - Walker, Peter B.

AU - Aligbe, Chioma

AU - Pappas, Georgina

AU - Tran Ho, Lam Thuy Vi

AU - Arnaud, Francoise G.

AU - Auker, Charles R.

AU - McCarron, Richard M.

AU - Scultetus, Anke H.

AU - Moon-Massat, Paula

PY - 2018/4

Y1 - 2018/4

N2 - Objective: The aim of this study was to assess, in two experiments, the safety and efficacy of the PFC emulsion Oxycyte as an oxygen therapeutic for TBI to test the hypothesis that early administration of this oxygen-carrying fluid post-TBI would improve brain tissue oxygenation (PbtO2). Methods: The first experiment assessed the effects of Oxycyte on cerebral vasoactivity in healthy, uninjured rats using intravital microscopy. The second experiment investigated the effect of Oxycyte on cerebral PbtO2 using the PQM in TBI model. Animals in the Oxycyte group received a single injection of Oxycyte (6 mL/kg) shortly after TBI, while NON animals received no treatment. Results: Oxycyte did not cause vasoconstriction in small- (<50 μm) or medium- (50-100 μm) sized pial arterioles nor did it cause a significant change in blood pressure. Treatment with Oxycyte while breathing 100% O2 did not improve PbtO2. However, in rats ventilated with ~40% O2, PbtO2 improved to near pre-TBI values within 105 minutes after Oxycyte injection. Conclusions: Although Oxycyte did not cause cerebral vasoconstriction, its use at the dose tested while breathing 100% O2 did not improve PbtO2 following TBI. However, Oxycyte treatment while breathing a lower enriched oxygen concentration may improve PbtO2 after TBI.

AB - Objective: The aim of this study was to assess, in two experiments, the safety and efficacy of the PFC emulsion Oxycyte as an oxygen therapeutic for TBI to test the hypothesis that early administration of this oxygen-carrying fluid post-TBI would improve brain tissue oxygenation (PbtO2). Methods: The first experiment assessed the effects of Oxycyte on cerebral vasoactivity in healthy, uninjured rats using intravital microscopy. The second experiment investigated the effect of Oxycyte on cerebral PbtO2 using the PQM in TBI model. Animals in the Oxycyte group received a single injection of Oxycyte (6 mL/kg) shortly after TBI, while NON animals received no treatment. Results: Oxycyte did not cause vasoconstriction in small- (<50 μm) or medium- (50-100 μm) sized pial arterioles nor did it cause a significant change in blood pressure. Treatment with Oxycyte while breathing 100% O2 did not improve PbtO2. However, in rats ventilated with ~40% O2, PbtO2 improved to near pre-TBI values within 105 minutes after Oxycyte injection. Conclusions: Although Oxycyte did not cause cerebral vasoconstriction, its use at the dose tested while breathing 100% O2 did not improve PbtO2 following TBI. However, Oxycyte treatment while breathing a lower enriched oxygen concentration may improve PbtO2 after TBI.

KW - controlled cortical impact

KW - intravital microscopy

KW - oxygen therapeutic

KW - phosphorescence quenching method

KW - pial microcirculation

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U2 - 10.1111/micc.12441

DO - 10.1111/micc.12441

M3 - Article

C2 - 29356218

AN - SCOPUS:85045391607

SN - 1073-9688

VL - 25

JO - Microcirculation

JF - Microcirculation

IS - 3

M1 - e12441

ER -

Brain oxygenation with a non-vasoactive perfluorocarbon emulsion in a rat model of traumatic brain injury

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Keywords

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