TY - JOUR
T1 - Burn injury is associated with an infiltration of the wound site with myeloid-derived suppressor cells
AU - Schwacha, Martin G.
AU - Scroggins, Shannon R.
AU - Montgomery, Robbie K.
AU - Nicholson, Susannah E.
AU - Cap, Andrew P.
N1 - Funding Information:
These findings were presented in part at the 41st annual meeting of the Shock Society in Scottsdale, AZ and the 2018 Military Health System Research Symposium in Kissimmee FL. This work was supported by funding from the William & Ella Owens Medical Research Foundation and the UTHSCSA Department of Surgery. The opinions or assertions contained herein are the private views of the author and are not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense. MGS was responsible for the scientific conception, experimental design and interpretation and drafting of the manuscript. SRS was responsible for in vitro experiments, flow cytometry, and data analysis. RKM assisted with experimental design and flow cytometry. SEN and APC were responsible for interpretation and assisted in drafting the manuscript. All authors read and approved the final manuscript.
Funding Information:
These findings were presented in part at the 41 st annual meeting of the Shock Society in Scottsdale, AZ and the 2018 Military Health System Research Symposium in Kissimmee FL. This work was supported by funding from the William & Ella Owens Medical Research Foundation and the UTHSCSA Department of Surgery. The opinions or assertions contained herein are the private views of the author and are not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/4
Y1 - 2019/4
N2 - Myeloid-derived suppressor cells (MDSCs) have been identified in the burn wound, however their characterization is incomplete. To study this, mice were subjected to a major burn and skin cells were isolated 3 days thereafter for analysis. Significant infiltration of the burn wound with MDSCs was observed as compared with uninjured skin. The skin of naïve mice did not contain MDSCs. Characterization of the cells showed that 33% of MDSCs in the wound were monocytic (M)-MDSCs, which was significantly less than that found in uninjured skin (52%). In contrast, polymorphonuclear (PMN)-MDSCs were greater in the burn wound as compared with uninjured skin. Burn wound TLR expression by both MDSCs subsets was decreased as compared with uninjured skin. Wound MDSCs produced pro- and anti-inflammatory cytokines and iNOS was present in both MDSC subsets, whereas ARG1 was only present in M-MDSCs. In conclusion, both M- and PMN-MDSCs infiltrate burn wound with after injury, however, they displayed decreased TLR expression, suggesting receptor down-regulation.
AB - Myeloid-derived suppressor cells (MDSCs) have been identified in the burn wound, however their characterization is incomplete. To study this, mice were subjected to a major burn and skin cells were isolated 3 days thereafter for analysis. Significant infiltration of the burn wound with MDSCs was observed as compared with uninjured skin. The skin of naïve mice did not contain MDSCs. Characterization of the cells showed that 33% of MDSCs in the wound were monocytic (M)-MDSCs, which was significantly less than that found in uninjured skin (52%). In contrast, polymorphonuclear (PMN)-MDSCs were greater in the burn wound as compared with uninjured skin. Burn wound TLR expression by both MDSCs subsets was decreased as compared with uninjured skin. Wound MDSCs produced pro- and anti-inflammatory cytokines and iNOS was present in both MDSC subsets, whereas ARG1 was only present in M-MDSCs. In conclusion, both M- and PMN-MDSCs infiltrate burn wound with after injury, however, they displayed decreased TLR expression, suggesting receptor down-regulation.
KW - ARG1
KW - Cytokines
KW - Inflammation
KW - iNOS
KW - Toll-like receptors
UR - http://www.scopus.com/inward/record.url?scp=85062915205&partnerID=8YFLogxK
U2 - 10.1016/j.cellimm.2019.03.001
DO - 10.1016/j.cellimm.2019.03.001
M3 - Article
C2 - 30902343
AN - SCOPUS:85062915205
SN - 0008-8749
VL - 338
SP - 21
EP - 26
JO - Cellular Immunology
JF - Cellular Immunology
ER -