Ca2+-dependent protein kinase 6 enhances kat2 shaker channel activity in arabidopsis thaliana

Elsa Ronzier, Claire Corratgé-Faillie, Frédéric Sanchez, Christian Brière, Tou Cheu Xiong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Post-translational regulations of Shaker-like voltage-gated K+ channels were reported to be essential for rapid responses to environmental stresses in plants. In particular, it has been shown that calcium-dependent protein kinases (CPKs) regulate Shaker channels in plants. Here, the focus was on KAT2, a Shaker channel cloned in the model plant Arabidopsis thaliana, where is it expressed namely in the vascular tissues of leaves. After co-expression of KAT2 with AtCPK6 in Xenopus laevis oocytes, voltage-clamp recordings demonstrated that AtCPK6 stimulates the activity of KAT2 in a calcium-dependent manner. A physical interaction between these two proteins has also been shown by Förster resonance energy transfer by fluorescence lifetime imaging (FRET-FLIM). Peptide array assays support that AtCPK6 phosphorylates KAT2 at several positions, also in a calcium-dependent manner. Finally, K+ fluorescence imaging in planta suggests that K+ distribution is impaired in kat2 knock-out mutant leaves. We propose that the AtCPK6/KAT2 couple plays a role in the homeostasis of K+ distribution in leaves.

Original languageEnglish
Article number1596
Pages (from-to)1-15
Number of pages15
JournalInternational Journal of Molecular Sciences
Volume22
Issue number4
DOIs
StatePublished - Feb 2021
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • CPK
  • Calcium signaling
  • Post-translational regulation
  • Shaker channel

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