CCL2-CCR2 signaling promotes hepatic ischemia/reperfusion injury

Junbin Zhang, Peng Xu, Peng Song, Hui Wang, Yong Zhang, Qinggang Hu, Guoliang Wang, Shu Zhang, Qilin Yu, Timothy R. Billiar, Congyi Wang*, Jinxiang Zhang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Background Liver ischemia/reperfusion (I/R) injury is a type of uncontrolled inflammatory cascade in which neutrophils, an early infiltrating immune cell population, elicit significant tissue damage. However, the precise mechanism for neutrophil recruitment and infiltration remains to be fully characterized. Methods A hepatic partial I/R model was reproduced in wild-type, CCL2-/- and CCR2-/- mice. Tissue damage was evaluated by serum enzyme analysis, hematoxylin-eosin staining, and cytokine production measurement. Mobilization of neutrophils from the bone marrow and subsequent infiltration into the liver were measured by flow cytometry. C-C motif chemokine receptor 2 (CCR2) expression on neutrophils and C-C motif chemokine ligand 2 (CCL2) chemotaxis were measured using flow cytometry. The cellular source of CCL2 in the liver was determined by deleting specific cell groups and performing intracellular staining. Results Liver damage was ameliorated, and neutrophil recruitment and accumulation were decreased in both CCL2-/- and CCR2-/- mice compared with wild-type mice. Neutrophils displayed upregulated expression of CCR2 during I/R, and these cells were required for CCL2-induced chemotaxis. Depletion of Kupffer cells protected the liver from I/R injury. Furthermore, genetic ablation of CCL2 reduced liver injury, as demonstrated by decreases in the levels of alanine aminotransferase and aspartate aminotransferase and subsequent reductions in neutrophil recruitment and accumulation. Conclusions Kupffer cells secrete CCL2 to promote CCR2-expressing neutrophil recruitment from the bone marrow and subsequent infiltration into the liver during I/R. These findings reveal a novel pro-inflammatory role of cell-mediated CCL2-CCR2 interactions during this sterile insult.

Original languageEnglish
Pages (from-to)352-362
Number of pages11
JournalJournal of Surgical Research
Volume202
Issue number2
DOIs
StatePublished - 15 May 2016
Externally publishedYes

Keywords

  • CCL2
  • CCR2
  • Ischemia/reperfusion
  • Kupffer cells
  • Neutrophils

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