CD38 expression on cryopreserved CD8+ T cells predicts HIV disease progression

Stephen P. Perfetto; John D. Malone; Clifton Hawkes; Gilbert McCrary; Bob August; Susan Zhou; Robin Garner; Matthew J. Dolan; Arthur E. Brown

doi:10.1002/(SICI)1097-0320(19981001)33:2<133::AID-CYTO7>3.0.CO;2-K

CD38 expression on cryopreserved CD8+ T cells predicts HIV disease progression

Stephen P. Perfetto^*, John D. Malone, Clifton Hawkes, Gilbert McCrary, Bob August, Susan Zhou, Robin Garner, Matthew J. Dolan, Arthur E. Brown

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Previous studies have revealed that the expression of CD38 on CD8⁺ T cells is a strong predictor of disease progression in human immunodeficiency virus (HIV)-infected individuals. Those studies were performed using fresh patient samples over an extended trim period. After demonstrating the validity of assay results on cryopreserved cells, we performed a retrospective study using frozen cell samples to determine the predictive value of CD38 expression in patients with CD4 counts above 400 cells/μl. The CD38 expression as measured by antibody binding capacity and the CD38 median channel were shown to be associated with time to new opportunistic infection or death (both P < 0.001). These results suggest that CD38 expression on CD8⁺ T cells, whether fresh or frozen, provides a useful predictor of HIV disease progression.

Original language	English
Pages (from-to)	133-137
Number of pages	5
Journal	Cytometry
Volume	33
Issue number	2
DOIs	https://doi.org/10.1002/(SICI)1097-0320(19981001)33:2<133::AID-CYTO7>3.0.CO;2-K
State	Published - 1 Oct 1998
Externally published	Yes

Keywords

Acquired immunodeficiency syndrome
CD38 antigen
Human immunodeficiency virus
Immunophenotyping
Prognosis
Relative fluorescence intensity

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10.1002/(SICI)1097-0320(19981001)33:2<133::AID-CYTO7>3.0.CO;2-K

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title = "CD38 expression on cryopreserved CD8+ T cells predicts HIV disease progression",

abstract = "Previous studies have revealed that the expression of CD38 on CD8+ T cells is a strong predictor of disease progression in human immunodeficiency virus (HIV)-infected individuals. Those studies were performed using fresh patient samples over an extended trim period. After demonstrating the validity of assay results on cryopreserved cells, we performed a retrospective study using frozen cell samples to determine the predictive value of CD38 expression in patients with CD4 counts above 400 cells/μl. The CD38 expression as measured by antibody binding capacity and the CD38 median channel were shown to be associated with time to new opportunistic infection or death (both P < 0.001). These results suggest that CD38 expression on CD8+ T cells, whether fresh or frozen, provides a useful predictor of HIV disease progression.",

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AU - Perfetto, Stephen P.

AU - Malone, John D.

AU - Hawkes, Clifton

AU - McCrary, Gilbert

AU - August, Bob

AU - Zhou, Susan

AU - Garner, Robin

AU - Dolan, Matthew J.

AU - Brown, Arthur E.

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AB - Previous studies have revealed that the expression of CD38 on CD8+ T cells is a strong predictor of disease progression in human immunodeficiency virus (HIV)-infected individuals. Those studies were performed using fresh patient samples over an extended trim period. After demonstrating the validity of assay results on cryopreserved cells, we performed a retrospective study using frozen cell samples to determine the predictive value of CD38 expression in patients with CD4 counts above 400 cells/μl. The CD38 expression as measured by antibody binding capacity and the CD38 median channel were shown to be associated with time to new opportunistic infection or death (both P < 0.001). These results suggest that CD38 expression on CD8+ T cells, whether fresh or frozen, provides a useful predictor of HIV disease progression.

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KW - Prognosis

KW - Relative fluorescence intensity

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CD38 expression on cryopreserved CD8+ T cells predicts HIV disease progression

Abstract

Keywords

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