TY - JOUR
T1 - Characterization and multilineage potential of cells derived from isolated microvascular fragments
AU - McDaniel, Jennifer S.
AU - Pilia, Marcello
AU - Ward, Catherine L.
AU - Pollot, Beth E.
AU - Rathbone, Christopher R.
N1 - Funding Information:
This study was supported by the US Army Medical Research and Materiel Command of the Department of Defense . The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or reflecting the views of the Department of Defense or US government. The authors would like to express our sincere gratitude to Monica Jalomo and Stephanie Roth for their invaluable technical assistance.
PY - 2014
Y1 - 2014
N2 - Background: A number of therapies are being developed that use microvessels isolated from adipose tissue (microvascular fragments [MVFs]) to improve tissue perfusion and implant survival. Because it has been demonstrated that stem cells are associated with microvessels, the purpose of these studies was to gain further insight into the stem cells associated with MVFs to better understand their therapeutic potential. Materials and methods: Cells derived from MVF explants were compared with adipose-derived stemcells (ASCs) based on the expression of cell surface proteins for mesenchymal stemcells and their capacity for angiogenic, neurogenic, adipogenic, and osteogenic differentiation. Results: The expression of cell surface proteins for mesenchymal stem cell markers was similar between MVF-derived cells and ASCs; however, the increase in markers consistent with endothelial cells and pericytes was accompanied by an improved ability to form capillary-like networks when cultured on matrigel. MVF-derived cells had increased neuregulin, leptin, and osteopontin expression compared with ASCs when exposed to neurogenic, adipogenic, and osteogenic induction media, respectively. Conclusions: The stem cell functionality of cells derived from MVFs is retained after their isolation. This helps to explain the ability of MVFs to improve tissue perfusion and has implications for the use of MVFs as a means to deliver stem cells within their niche.
AB - Background: A number of therapies are being developed that use microvessels isolated from adipose tissue (microvascular fragments [MVFs]) to improve tissue perfusion and implant survival. Because it has been demonstrated that stem cells are associated with microvessels, the purpose of these studies was to gain further insight into the stem cells associated with MVFs to better understand their therapeutic potential. Materials and methods: Cells derived from MVF explants were compared with adipose-derived stemcells (ASCs) based on the expression of cell surface proteins for mesenchymal stemcells and their capacity for angiogenic, neurogenic, adipogenic, and osteogenic differentiation. Results: The expression of cell surface proteins for mesenchymal stem cell markers was similar between MVF-derived cells and ASCs; however, the increase in markers consistent with endothelial cells and pericytes was accompanied by an improved ability to form capillary-like networks when cultured on matrigel. MVF-derived cells had increased neuregulin, leptin, and osteopontin expression compared with ASCs when exposed to neurogenic, adipogenic, and osteogenic induction media, respectively. Conclusions: The stem cell functionality of cells derived from MVFs is retained after their isolation. This helps to explain the ability of MVFs to improve tissue perfusion and has implications for the use of MVFs as a means to deliver stem cells within their niche.
KW - Angiogenesis
KW - Mesenchymal stem cell
KW - Microvessel
UR - http://www.scopus.com/inward/record.url?scp=84921737295&partnerID=8YFLogxK
U2 - 10.1016/j.jss.2014.05.047
DO - 10.1016/j.jss.2014.05.047
M3 - Article
C2 - 24969547
AN - SCOPUS:84921737295
SN - 0022-4804
VL - 192
SP - 214
EP - 222
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 1
ER -