Characterization of Norwalk-like virus associated with gastroenteritis in Indonesia

D. S. Subekti; P. Tjaniadi; M. Lesmana; C. Simanjuntak; S. Komalarini; H. Digdowirogo; B. Setiawan; A. L. Corwin; J. R. Campbell; K. R. Porter; B. A. Oyofo

doi:10.1002/jmv.2215

Characterization of Norwalk-like virus associated with gastroenteritis in Indonesia

D. S. Subekti, P. Tjaniadi, M. Lesmana, C. Simanjuntak, S. Komalarini, H. Digdowirogo, B. Setiawan, A. L. Corwin, J. R. Campbell, K. R. Porter, B. A. Oyofo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Norwalk Virus and Norwalk-like viruses (NLVs) are reportedly responsible for 2.5-4.0% of nonbacterial acute gastroenteritis (NBAG) worldwide. To help clarify the impact of NLVs on NBAG in Indonesia, stool specimens from 102 patients, 74 with NBAG and 28 with BAG, were screened for the presence of NLVs, using a reverse transcription-polymerase chain reaction (RT-PCR) assay. The specimens were subtyped using prototype-specific oligonucleotide probes and were sequenced and compared with published NLV sequences. Of the 102 specimens examined, 31 (30%) were found to be positive for NLVs. Type-specific probe analysis of the RT-PCR products indicated that 31 isolates hybridized to UK1 (Taunton agent) and UK3/4 (Hawaii agent/Snow Mountain agent) prototype strains. The results of this study indicate that prototype strains of NV or NLVs co-circulate in Indonesia and contribute to the overall level of acute gastroenteritis throughout the region.

Original language	English
Pages (from-to)	253-258
Number of pages	6
Journal	Journal of Medical Virology
Volume	67
Issue number	2
DOIs	https://doi.org/10.1002/jmv.2215
State	Published - 2002
Externally published	Yes

Keywords

Diarrhea
Norwalk-like viruses (NLVs)
RT-PCR
Sequence

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title = "Characterization of Norwalk-like virus associated with gastroenteritis in Indonesia",

abstract = "Norwalk Virus and Norwalk-like viruses (NLVs) are reportedly responsible for 2.5-4.0% of nonbacterial acute gastroenteritis (NBAG) worldwide. To help clarify the impact of NLVs on NBAG in Indonesia, stool specimens from 102 patients, 74 with NBAG and 28 with BAG, were screened for the presence of NLVs, using a reverse transcription-polymerase chain reaction (RT-PCR) assay. The specimens were subtyped using prototype-specific oligonucleotide probes and were sequenced and compared with published NLV sequences. Of the 102 specimens examined, 31 (30%) were found to be positive for NLVs. Type-specific probe analysis of the RT-PCR products indicated that 31 isolates hybridized to UK1 (Taunton agent) and UK3/4 (Hawaii agent/Snow Mountain agent) prototype strains. The results of this study indicate that prototype strains of NV or NLVs co-circulate in Indonesia and contribute to the overall level of acute gastroenteritis throughout the region.",

keywords = "Diarrhea, Norwalk-like viruses (NLVs), RT-PCR, Sequence",

author = "Subekti, {D. S.} and P. Tjaniadi and M. Lesmana and C. Simanjuntak and S. Komalarini and H. Digdowirogo and B. Setiawan and Corwin, {A. L.} and Campbell, {J. R.} and Porter, {K. R.} and Oyofo, {B. A.}",

year = "2002",

doi = "10.1002/jmv.2215",

language = "English",

volume = "67",

pages = "253--258",

journal = "Journal of Medical Virology",

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AU - Subekti, D. S.

AU - Tjaniadi, P.

AU - Lesmana, M.

AU - Simanjuntak, C.

AU - Komalarini, S.

AU - Digdowirogo, H.

AU - Setiawan, B.

AU - Corwin, A. L.

AU - Campbell, J. R.

AU - Porter, K. R.

AU - Oyofo, B. A.

PY - 2002

Y1 - 2002

N2 - Norwalk Virus and Norwalk-like viruses (NLVs) are reportedly responsible for 2.5-4.0% of nonbacterial acute gastroenteritis (NBAG) worldwide. To help clarify the impact of NLVs on NBAG in Indonesia, stool specimens from 102 patients, 74 with NBAG and 28 with BAG, were screened for the presence of NLVs, using a reverse transcription-polymerase chain reaction (RT-PCR) assay. The specimens were subtyped using prototype-specific oligonucleotide probes and were sequenced and compared with published NLV sequences. Of the 102 specimens examined, 31 (30%) were found to be positive for NLVs. Type-specific probe analysis of the RT-PCR products indicated that 31 isolates hybridized to UK1 (Taunton agent) and UK3/4 (Hawaii agent/Snow Mountain agent) prototype strains. The results of this study indicate that prototype strains of NV or NLVs co-circulate in Indonesia and contribute to the overall level of acute gastroenteritis throughout the region.

AB - Norwalk Virus and Norwalk-like viruses (NLVs) are reportedly responsible for 2.5-4.0% of nonbacterial acute gastroenteritis (NBAG) worldwide. To help clarify the impact of NLVs on NBAG in Indonesia, stool specimens from 102 patients, 74 with NBAG and 28 with BAG, were screened for the presence of NLVs, using a reverse transcription-polymerase chain reaction (RT-PCR) assay. The specimens were subtyped using prototype-specific oligonucleotide probes and were sequenced and compared with published NLV sequences. Of the 102 specimens examined, 31 (30%) were found to be positive for NLVs. Type-specific probe analysis of the RT-PCR products indicated that 31 isolates hybridized to UK1 (Taunton agent) and UK3/4 (Hawaii agent/Snow Mountain agent) prototype strains. The results of this study indicate that prototype strains of NV or NLVs co-circulate in Indonesia and contribute to the overall level of acute gastroenteritis throughout the region.

KW - Diarrhea

KW - Norwalk-like viruses (NLVs)

KW - RT-PCR

KW - Sequence

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SN - 0146-6615

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SP - 253

EP - 258

JO - Journal of Medical Virology

JF - Journal of Medical Virology

IS - 2

ER -

Characterization of Norwalk-like virus associated with gastroenteritis in Indonesia

Abstract

Keywords

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