Chemoattraction of progenitor cells by remodeling extracellular matrix scaffolds

Allison J. Beattie, Thomas W. Gilbert, Juan Pablo Guyot, Adolph J. Yates, Stephen F. Badylak

Research output: Contribution to journalArticlepeer-review

189 Scopus citations

Abstract

The chemotactic properties of a biologic scaffold composed of extracellular matrix (ECM) and subjected to in vivo degradation and remodeling were evaluated in a mouse model of Achilles tendon reconstruction. Following a segmental resection of the Achilles tendon in both C57BL/6 and MRL/MpJ mice, the defect was repaired with either an ECM scaffold composed of urinary bladder matrix (UBM) or resected autologous tendon. The surgically repaired and the contralateral tendons were harvested at 3, 7, and 14 days following surgery from each animal. Chemotaxis of multipotential progenitor cells toward the harvested tissue was quantified using a fluorescent-based cell migration assay. Results showed greater migration of progenitor cells toward tendons repaired with UBM-ECM scaffold compared to both the tendons repaired with autologous tissue and the normal contralateral tendon in both the MRL/MpJ and C57BL/6 mice. The magnitude and temporal pattern of the chemotactic response differed between the two mouse strains.

Original languageEnglish
Pages (from-to)1119-1125
Number of pages7
JournalTissue Engineering - Part A.
Volume15
Issue number5
DOIs
StatePublished - 1 May 2009
Externally publishedYes

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